TWEEN 20 BioXtra, viscous liquid, 500mL

SKU:
P7949-500ML
  • $83.30

    • Description

    TWEEN 20 BioXtra, viscous liquid, 500mL

    Synonym: Polyethylene glycol sorbitan monolaurate, Polyoxyethylenesorbitan monolaurate

    • CAS Number 9005-64-5

       

    •  MDL number MFCD00165986

       

    •  NACRES NA.21

    Properties

    Related Categories Antigen-Vaccine Preparation, Biochemicals and Reagents, Cell Culture, Core Bioreagents, Detergents,
    Detergents A to M, Detergents by Applications, Diagnostic Applications, Drug Delivery-Liposomes, Electrophoresis Chromatography, Enzymology, Membrane Protein Solubilization, Non-Ionic Detergents, Research Essentials
    Less...
    Quality Level 300
    description non-ionic
    product line BioXtra
    form viscous liquid
    mol wt ~1228
    composition lauric acid, ≥40% (balance primarily myristic, palmoyic, and stearic acids)
    application(s) blocking: suitable
     - cell culture | mammalian: suitable
     - hybridization: suitable
     - protein purification: suitable
     - transformation: suitable
    impurities ≤0.05% Phosphorus (P)
    ign. residue ≤0.2%
    refractive index n20/D 1.468 (lit.)
    CMC 0.06 mM (20-25°C)
    transition temp cloud point 76 °C
    density 1.095 g/mL at 25 °C (lit.)
    anion traces chloride (Cl-): ≤0.05%
     -  sulfate (SO42-): ≤0.05%
    cation traces Al: ≤0.0005%
     - Ca: ≤0.0005%
     - Cu: ≤0.0005%
     - Fe: ≤0.0005%
     - K: ≤0.005%
     - Mg: ≤0.0005%
     - NH4+: ≤0.05%
     - Na: ≤0.05%
     - Pb: ≤0.001%
     - Zn: ≤0.0005%
    HLB 16.7
    InChI 1S/C26H50O10/c1-2-3-4-5-6-7-8-9-10-11-24(30)34-19-18-31-20-22(32-15-12-27)26-25(35-17-14-29)23(21-36-26)33-16-13-28/h22-23,25-29H,2-21H2,1H3
    InChI key HMFKFHLTUCJZJO-UHFFFAOYSA-N

    General description

    Tween®20 is a polyoxyethylene sorbitol ester that belongs to the polysorbate family. It is a nonionic detergent having a molecular weight of 1,225 daltons, assuming 20 ethylene oxide units, 1 sorbitol, and 1 lauric acid as the primary fatty acid. The ethylene oxide subunits are responsible for the hydrophilic nature of the surfactant, while the hydrocarbon chains provide the hydrophobic environment. Sorbitol forms the backbone ring to which the ethylene oxide polymers are attached.[1]

    Application

    TWEEN 20 has been used in post-hybridization wash buffer after in situ hybridization.[2] It has been used along with PBS (phosphate buffered saline) to wash wells during ELISA[3][4] and along with PBS (phosphate buffered saline) in wash buffer for immunostaining.[5]

    TWEEN 20 is a nonionic detergent widely used in biochemical applications. It has been used as an emulsifying agent for the preparation of stable oil-in-water emulsions. TWEEN 20 has been used in pre-extraction of membranes to remove peripheral proteins (used at 2% for extraction of membrane-bound proteins).
    TWEEN 20 has been used as a blocking agent for membrane based immunoassays at a typical concentration of 0.05%. TWEEN 20 can be used for lysing mammalian cells at a concentration of 0.005 to 0.5%.

    Non-ionic detergent

    Packaging

    100, 500 mL in poly bottle

    Caution

    TWEEN 20 may not be stable to autoclaving, particularly with metal cations in buffer solutions. Autoclaving is not recommended without testing for changes in properties. TWEEN 20 is heat sensitive and will darken when exposed to elevated temperatures. Polysorbates have been reported to be incompatible with alkalis, heavy metal salts, phenols, and tannic acid. They may also reduce the activity of many preservatives. Aqueous solutions of polysorbates undergo autoxidation during storage, with changes being catalyzed by light, increased temperature, and copper sulfate.

    Preparation Note

    TWEEN 20 is miscible in water (100mg/mL), yielding a clear, yellow solution. It is also miscible with alcohol, dioxane, and ethyl acetate. TWEEN 20 is insoluble in liquid paraffin and fixed oils.

    Legal Information

    TWEEN is a registered trademark of Croda International PLC

    Safety Information

    Personal Protective Equipment 
    Eyeshields, Gloves
    RIDADR 
    NONH for all modes of transport
    WGK Germany 
    WGK 1
    RTECS 
    TR7400000
    Flash Point(F) 
    527.0 °F - Pensky-Martens closed cup
    Flash Point(C) 
    275 °C - Pensky-Martens closed cup