TWEEN 20 100mL

SKU:
P1379-100ML
  • $38.00

    • Description

    TWEEN® 20

     

    viscous liquid

    Synonym: Polyethylene glycol sorbitan monolaurate, Polyoxyethylenesorbitan monolaurate

    • CAS Number 9005-64-5

    •  MDL number MFCD00165986

    Properties

    Related Categories Antigen-Vaccine Preparation, Biochemicals and Reagents, Buffers, Cell Biology, Cell Culture,
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    Quality Level   PREMIUM
    description   non-ionic
    form   viscous liquid
    mol wt   mol wt ~1228
    composition   lauric acid, ≥40% (balance primarily myristic, palmitic, and stearic acids)
    impurities   ≤3.0% water
    refractive index   n20/D 1.468(lit.)
    CMC   0.06 mM(20-25°C)
    acid number   ≤2.2 mg/g
    hydroxyl value   96‑108 mg/g
    transition temp   cloud point 76 °C
    density   1.095 g/mL at 25 °C(lit.)
    HLB   16.7
    suitability   complies for IR spectroscopy

    Description

    Application

    Non-ionic detergent

    TWEEN 20 has been used as a component of:
    • washing buffer and blocking buffer in Western blotting[2]
    • blocking buffer in immunohistochemistry[3]
    • washing buffer in ELISA (enzyme linked immunosorbent assay)[4][1]
    • reaction mixture in PCR (polymerase chain reaction)[5]

    TWEEN 20 is a nonionic detergent widely used in biochemical applications. It has been used as an emulsifying agent for the preparation of stable oil-in-water emulsions. TWEEN 20 has been used in pre-extraction of membranes to remove peripheral proteins (used at 2% for extraction of membrane-bound proteins). 
    TWEEN 20 has been used as a blocking agent for membrane based immunoassays at a typical concentration of 0.05%. TWEEN 20 can be used for lysing mammalian cells at a concentration of 0.005 to 0.5%.

    Packaging

    1, 4 L in poly bottle

    25, 100, 250, 500, 6×500 mL in poly bottle

    Preparation Note

    TWEEN 20 is miscible in water (100mg/mL), yielding a clear, yellow solution. It is also miscible with alcohol, dioxane, and ethyl acetate. TWEEN 20 is insoluble in liquid paraffin and fixed oils.

    General description

    Tween®20 is a polyoxyethylene sorbitol ester that belongs to the polysorbate family. It is a nonionic detergent having a molecular weight of 1,225 daltons, assuming 20 ethylene oxide units, 1 sorbitol, and 1 lauric acid as the primary fatty acid. The ethylene oxide subunits are responsible for the hydrophilic nature of the surfactant, while the hydrocarbon chains provide the hydrophobic environment. Sorbitol forms the backbone ring to which the ethylene oxide polymers are attached.[6]

    Legal Information

    TWEEN is a registered trademark of Croda International PLC