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TWEEN 20, 1L

SKU:
P1379-1L
  • $90.30

    • Description

    TWEEN® 20, 1L

    viscous liquid

    Synonym(s):
    Polyethylene glycol sorbitan monolaurate, Polyoxyethylenesorbitan monolaurate
    CAS Number:
    9005-64-5
    MDL number:
    MFCD00165986
    NACRES:
    NA.21

    PROPERTIES

    description

    non-ionic

    Quality Level

    300

    assay

    ≥40.0% (GC)

    form

    viscous liquid

    mol wt

    ~1228

    composition

    lauric acid, ≥40% (balance primarily myristic, palmitic, and stearic acids)

    technique(s)

    DNA extraction: suitable
    RNA extraction: suitable
    cell culture | mammalian: suitable
    protein purification: suitable

    impurities

    ≤3.0% water

    refractive index

    n20/D 1.468 (lit.)

    CMC

    0.06 mM (20-25°C)

    acid number

    ≤2.2 mg/g

    hydroxyl value

    96‑108 mg/g

    transition temp

    cloud point 76 °C

    density

    1.095 g/mL at 25 °C (lit.)

    HLB

    16.7

    suitability

    complies for IR spectroscopy

    InChI

    1S/C26H50O10/c1-2-3-4-5-6-7-8-9-10-11-24(30)34-19-18-31-20-22(32-15-12-27)26-25(35-17-14-29)23(21-36-26)33-16-13-28/h22-23,25-29H,2-21H2,1H3

    InChI key

    HMFKFHLTUCJZJO-UHFFFAOYSA-N

    General description

    Tween®20 is a polyoxyethylene sorbitol ester that belongs to the polysorbate family. It is a nonionic detergent having a molecular weight of 1,225 daltons, assuming 20 ethylene oxide units, 1 sorbitol, and 1 lauric acid as the primary fatty acid. The ethylene oxide subunits are responsible for the hydrophilic nature of the surfactant, while the hydrocarbon chains provide the hydrophobic environment. Sorbitol forms the backbone ring to which the ethylene oxide polymers are attached.

    Application

    TWEEN 20 has been used as a component of:
    • washing buffer and blocking buffer in Western blotting
    • blocking buffer in immunohistochemistry
    • washing buffer in ELISA (enzyme linked immunosorbent assay)
    • reaction mixture in PCR (polymerase chain reaction)
    TWEEN 20 is a nonionic detergent widely used in biochemical applications. It has been used as an emulsifying agent for the preparation of stable oil-in-water emulsions. TWEEN 20 has been used in pre-extraction of membranes to remove peripheral proteins (used at 2% for extraction of membrane-bound proteins).
    TWEEN 20 has been used as a blocking agent for membrane based immunoassays at a typical concentration of 0.05%. TWEEN 20 can be used for lysing mammalian cells at a concentration of 0.005 to 0.5%.
    Non-ionic detergent

    Packaging

    25, 100, 250, 500, 6×500 mL in poly bottle
    1, 4 L in poly bottle
    P1379-1KT:
    Each kit contains 3 x 100ML samples, each sample from a uniquely manufactured lot.

    Preparation Note

    TWEEN 20 is miscible in water (100mg/mL), yielding a clear, yellow solution. It is also miscible with alcohol, dioxane, and ethyl acetate. TWEEN 20 is insoluble in liquid paraffin and fixed oils.

    Legal Information

    TWEEN is a registered trademark of Croda International PLC