Description
TWEEN® 20
viscous liquid
Synonym: Polyethylene glycol sorbitan monolaurate, Polyoxyethylenesorbitan monolaurate
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CAS Number 9005-64-5
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MDL number MFCD00165986
Properties
Related Categories | Antigen-Vaccine Preparation, Biochemicals and Reagents, Buffers, Cell Biology, Cell Culture, |
Quality Level | PREMIUM |
description | non-ionic |
form | viscous liquid |
mol wt | mol wt ~1228 |
composition | lauric acid, ≥40% (balance primarily myristic, palmitic, and stearic acids) |
impurities | ≤3.0% water |
refractive index | n20/D 1.468(lit.) |
CMC | 0.06 mM(20-25°C) |
acid number | ≤2.2 mg/g |
hydroxyl value | 96‑108 mg/g |
transition temp | cloud point 76 °C |
density | 1.095 g/mL at 25 °C(lit.) |
HLB | 16.7 |
suitability | complies for IR spectroscopy |
Description
Application
Non-ionic detergent
TWEEN 20 has been used as a component of:
• washing buffer and blocking buffer in Western blotting[2]
• blocking buffer in immunohistochemistry[3]
• washing buffer in ELISA (enzyme linked immunosorbent assay)[4][1]
• reaction mixture in PCR (polymerase chain reaction)[5]
TWEEN 20 is a nonionic detergent widely used in biochemical applications. It has been used as an emulsifying agent for the preparation of stable oil-in-water emulsions. TWEEN 20 has been used in pre-extraction of membranes to remove peripheral proteins (used at 2% for extraction of membrane-bound proteins).
TWEEN 20 has been used as a blocking agent for membrane based immunoassays at a typical concentration of 0.05%. TWEEN 20 can be used for lysing mammalian cells at a concentration of 0.005 to 0.5%.
Packaging
1, 4 L in poly bottle
25, 100, 250, 500, 6×500 mL in poly bottle
Preparation Note
TWEEN 20 is miscible in water (100mg/mL), yielding a clear, yellow solution. It is also miscible with alcohol, dioxane, and ethyl acetate. TWEEN 20 is insoluble in liquid paraffin and fixed oils.
General description
Tween®20 is a polyoxyethylene sorbitol ester that belongs to the polysorbate family. It is a nonionic detergent having a molecular weight of 1,225 daltons, assuming 20 ethylene oxide units, 1 sorbitol, and 1 lauric acid as the primary fatty acid. The ethylene oxide subunits are responsible for the hydrophilic nature of the surfactant, while the hydrocarbon chains provide the hydrophobic environment. Sorbitol forms the backbone ring to which the ethylene oxide polymers are attached.[6]
Legal Information
TWEEN is a registered trademark of Croda International PLC