Trizma Pre-set crystals, 100G

SKU:
T9693-100G
  • $129.00

    • Description

    Trizma® Pre-set crystals

    BioPerformance Certified, pH 9.0, average Mw 124.6

    Synonym(s):
    Tris crystals

    PROPERTIES

    grade

    BioPerformance Certified

    Quality Level

    200

    form

    crystalline powder

    mol wt

    average Mw 124.6

    technique(s)

    cell culture | mammalian: suitable

    impurities

    DNase, RNase, Protease, NICKase, none detected
    endotoxin, tested
    total aerobic microbial count, tested
    ≤0.05 A290
    ≤0.5% water (Karl Fischer)
    ≤5 ppm Heavy metals (as Pb)

    pH

    9.00 (25 °C, 0.1 M)(lit.)
    9.0

    useful pH range

    7.0-9.0

    solubility

    water: 0.66 g/mL, clear, colorless

    application(s)

    diagnostic assay manufacturing

    DESCRIPTION

    Application

    Dissolve in water to make a 0.1M solution. No pH adjustment is necessary. Guaranteed accuracy: ±0.1 pH units.

    Tris has a significant temperature coefficient, so select the mixture to give the desired final pH at the desired temperature.
    From 5 °C to 25 °C, the pH decreases an average of 0.03 pH units per °C.
    From 25 °C to 37 °C, the pH decreases an average of 0.025 pH units per °C.

    If a different concentration is desired, note that there is a concentration effect on the pH of the solution.
    The product is cell culture tested. It is also tested for low endotoxin levels and analyzed for the absence of nucleases and proteases.

    Other Notes

    This product is a pre-mixed combination of Trizma base and Trizma HCl that can be used to prepare a common pH value for Tris buffer.
    Certain pH electrodes do not give accurate results with Tris buffers.

    Preparation Note

    Trizma solutions can be autoclaved.

    Legal Information

    Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

    SAFETY INFORMATION

    Storage Class Code

    11 - Combustible Solids

    WGK

    WGK 1

    Flash Point(F)

    Not applicable

    Flash Point(C)

    Not applicable

    Personal Protective Equipment

    dust mask type N95 (US), Eyeshields, Gloves