Triton X-100, 50ML

SKU:
T8787-50ML
  • $36.60

    • Description

    Triton™ X-100

    for molecular biology

    Synonym(s):
    t-Octylphenoxypolyethoxyethanol, Polyethylene glycol tert-octylphenyl ether
    Linear Formula:
    t-Oct-C6H4-(OCH2CH2)xOH, x= 9-10
    CAS Number:
    9036-19-5
    Beilstein:
    2315025
    MDL number:
    MFCD00128254
    PubChem Substance ID:
    329826885
    NACRES:
    NA.21

    PROPERTIES

    grade

    for molecular biology

    description

    non-ionic

    form

    liquid

    mol wt

    micellar avg mol wt 80,000
    average mol wt 625

    aggregation number

    100-155

    technique(s)

    protein purification: suitable
    protein quantification: suitable
    western blot: suitable

    pH

    9.7

    CMC

    0.2-0.9 mM (20-25°C)

    mp

    6 °C

    transition temp

    cloud point 65 °C
    pour point ~7 °C

    solubility

    water: 0.1 mL/mL, clear to slightly hazy, colorless to faintly yellow

    density

    1.06 g/mL at 25 °C (lit.)

    HLB

    13.5

    application(s)

    hematology
    histology

    foreign activity

    DNase and RNase, none detected

    storage temp.

    room temp

    SMILES string

    CC(C)(C)CC(C)(C)c1ccc(OCCOCCOCCOCCOCCOCCOCCO)cc1

    InChI

    1S/C28H50O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h6-9,29H,10-24H2,1-5H3

    InChI key

    HNLXNOZHXNSSPN-UHFFFAOYSA-N

    DESCRIPTION

    General description

    Triton™ X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection.[1][2]

    Application

    Triton™ X-100 has been used:
    • In immunohistochemistry for staining the Flat-mount retinas[3]
    • Along with ice-cold PBS (phosphate buffered saline) in suspension of cells for cell DNA analysis and Annexin V assay[4]
    • To permeabilise cells during Immunofluorescent microscopic studies[5]
    • As a positive control in LDH assay to determine the cell membrane integrity[6]
    • For estimating the lipase activity in postheparin plasma by using modified Belfrage and Vaughan radioenzymatic procedure[7]
    • For the preparation of outer membrane protein exctract[8]
    • As a component of extraction buffer along with tris-HCl, NaCl, CaCl2, ZnCl2, Brij 35 for homogenization of mice lung cells[9]
    • In the treatment of tissue sections for Immunofluorescence labeling[10]

    Biochem/physiol Actions

    Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.

    Legal Information

    Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

    SAFETY INFORMATION

    Signal Word

    Danger

    Hazard Statements

    H302 - H315 - H318 - H410

    Precautionary Statements

    P264 - P273 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338

    Hazard Classifications

    Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2

    Storage Class Code

    10 - Combustible liquids

    WGK

    WGK 3

    Flash Point(F)

    483.8 °F

    Flash Point(C)

    251 °C

    Personal Protective Equipment

    dust mask type N95 (US), Eyeshields, Gloves