JumpStart Taq ReadyMix for High Throughput Quantitative PCR Ready-to-use 2x mix for qPCR with ROX, 20 reactions

SKU:
D6442-20RXN
  • $54.60

    • Description

    JumpStart Taq ReadyMix for High Throughput Quantitative PCR

    Ready-to-use 2x mix for qPCR with ROX, 20 reactions

    Properties

    Related Categories Amplification, Cell Biology, Functional Profiling of Stem Cells, General Stem Cell Biology, Molecular Biology,
    Nucleic Acid Amplification, PCR/Amplification, Plant Biotechnology, Plant Molecular Biology, Probe Based Quantitative PCR, Quantitative PCR, Stem Cell Biology, Stem Cell Characterization, Traditional QPCR
    Quality Level 200
    form liquid
    feature hotstart
    concentration 2.5 units/reaction (50 μL reaction volume)
    color colorless
    Featured Industry Agriculture
    compatibility for use with ABI 5700
    - for use with ABI 7000
    -  for use with ABI 7300
    -  for use with ABI 7500 Fast
    -  for use with ABI 7500
    -  for use with ABI 7700
    -  for use with ABI 7900 Fast
    -  for use with ABI 7900 HT
    -  for use with ABI 7900
    -  for use with ABI StepOne
    -  for use with ABI StepOnePlus
    -  for use with ABI ViiA 7
    -  for use with Bio-Rad CFX384
    -  for use with Bio-Rad CFX96
    -  for use with Bio-Rad MJ Chromo4
    -  for use with Bio-Rad MJ Opticon 2
    -  for use with Bio-Rad MJ Opticon Cepheid SmartCycler
    -  for use with Bio-Rad MJ Opticon
    -  for use with Bio-Rad MiniOpticon
    -  for use with Eppendorf® Mastercycler ep realplex2 s
    -  for use with Eppendorf® Mastercycler ep realplex
    -  for use with Illumina Eco qPCR
    -  for use with Qiagen Corbett Rotor-Gene 3000
    -  for use with Qiagen Corbett Rotor-Gene 6000
    -  for use with Qiagen Corbett Rotor-Gene Q
    -  for use with Roche LightCycler 480
    -  for use with Strategene Mx3000P
    -  for use with Strategene Mx3005P
    -  for use with Strategene Mx4000
    shipped in wet ice
    storage temp. −20°C

    Application

    • For qPCR amplifications using SYBR
    • For multiplex PCR
    • For reduction of primer dimers

    Features and Benefits

    • To prepare a typical QPCR reaction, mix 25 μL of JumpStart ReadyMix Taq, fluorescent probe, desired amount of magnesium chloride (above 1.5 mM final concentration), template DNA, and primers in a final volume of 50 μL . Reaction volumes can be scaled down, if desired.
    • JumpStart Taq DNA polymerase prevents non-specific product formation, and allows assembled PCR reactions to be placed at room temperature for up to two hours without compromising the performance.
    • Internal Reference Dye is provided for reaction normalization.
    • When performing large numbers of PCR reactions, JumpStart ReadyMix Taq can save a significant amount of preparation time, reduce the risk of contamination from multiple pipetting steps, and provide consistent batch-to-batch and reaction-to-reaction performance.

    Packaging

    Default reaction volume is 50 μL

    20RXN is packaged as 1 X 500 μL
    400RXN is packaged as 1 X 10 mL
    2000RXN is packaged as 1 X 50 mL

    Other Notes

    JumpStart Taq ReadyMix For High Throughput Quantitative PCR combines the performance enhancements of JumpStart Taq Antibody for hot start PCR with the convenience of an easy-to-use reaction mixture that incorporates the internal reference dye for ABI and other real time instrument applications. The ReadyMix contains JumpStart Taq DNA polymerase, 99% pure deoxynucleotides and reaction buffer. It is provided in a 2× concentrate. Simply add an equal volume of the 2× ReadyMix to a 2× mixture DNA template, primers and fluorescent probe.

    JumpStart Taq ReadyMix for High Throughput Quantitative PCR is a reaction formulation used for high throughput hot-start quantitative PCR methods. It contains the hot start DNA polymerase reaction mixture with ROX dye as an internal reference for normalization of reactions.

    Quantity

    Use 2.5 units per reaction.

    Legal Information

    Eppendorf is a registered trademark of Eppendorf AG

    JumpStart is a trademark of Sigma-Aldrich Co. LLC

    ReadyMix is a trademark of Sigma-Aldrich Co. LLC

    Safety Information

    Symbol 
    GHS03,GHS05,GHS07,GHS09
    Signal word 
    Danger
    Hazard statements 
    H272 - H315 - H317 - H318 - H410
    Precautionary statements 
    P210 - P273 - P280 - P302 + P352 - P305 + P351 + P338 + P310
    RIDADR 
    UN 3082 9 / PGIII