MilliporeSigma
HEPES solution 1 M, pH 7.0-7.6, sterile-filtered, BioReagent (100mL)
- SKU:
- H0887-100ML
Description
HEPES solution
1 M, pH 7.0-7.6, sterile-filtered, BioReagent, suitable for cell culture
Synonym: N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
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CAS Number 7365-45-9
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MDL number MFCD00006158
Properties
Quality Level | PREMIUM |
sterility | sterile-filtered |
product line | BioReagent |
form | liquid |
concentration | 1 M |
impurities | endotoxin, tested |
pH | 7.0-7.6 |
useful pH range | 6.8 - 8.2 |
pH range | 7.0 - 7.6 |
suitability | suitable for cell culture |
storage temp. | 2-8°C |
Description
Caution
Concentrations of HEPES over 40mM may be toxic to some cell types.
Preparation Note
Prepared from HEPES free acid and cell culture grade water.
Application
HEPES is an organic zwitterionic buffering agent effective in the physiological pH range of 6.8 to 8.2 (pKa 7.55). It is typically used in cell culture at concentration between 5mM to 30 mM. This product has been qualified for use in cell culture.