Description
Imidazole buffer Solution
BioUltra, 1 M in H2O
Synonym: Glyoxaline solution, Imidazole solution
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CAS Number 288-32-4
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Empirical Formula (Hill Notation) C3H4N2
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Molecular Weight 68.08
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Beilstein Registry Number 103853
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MDL number MFCD00005183
Properties
| product line | BioUltra |
| concentration | 1 M in H2O |
| impurities | DNases, none detected |
| - | RNases, none detected |
| - | insoluble matter, passes filter test |
| - | phosphatases, none detected |
| - | proteases, none detected |
| pH | 9.5±0.5 |
| density | 1.01 g/mL at 20 °C |
Description
Application
Imidazole can be used as a buffer in the pH range of 6.2-7.8 at 25°C. It is recommended as a component of a buffer for assay of horseradish peroxidase and as chelator for the binding of various divalent cations. Imidazole can be used for the elution of histidine containing proteins from divalent cation resins (Sigma P6611, HisSelect.-HC Nickel affinity gel) and can also be used in reverse staining of SDS-PAGE gels for detection of proteins.
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