Description
DRBC (Dichloran-rose bengal chloramphenicol) agar
acc. ISO 21527 and FDA-BAM GranuCult
PROPERTIES
Agency
ISO
Quality Level
200
reg. compliance
FDA
form
medium granules (dehydrated (DCM))
manufacturer/tradename
GranuCult®
technique(s)
microbiological culture: suitable
pH
5.4-5.8 (25 °C, 32.6 g/L in H2O)
solubility
32.6 g/L
application(s)
food and beverages
microbiology
storage temp.
15-25°C
suitability
molds
yeasts
General description
GranuCult is our new brand name for our existing granulated dehydrated culture media which apply to new EN ISO 11133:2014 and various other ISO standards and FDA-BAM / USDA-FSIS methods for testing of food and water samples. All these media are currently being updated to be fully compliant to the latest version of these standards and regulations with respect to composition and quality control and will be available shortly.
Packaging
500 g in Plastic bottle
Analysis Note
Appearance (clearness): clear to slightly opalescent
Appearance (colour): pink
pH-value (25 °C): 5.4 - 5.8
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Inoculum on reference medium (Saccharomyces cerevisiae ATCC 9763 (WDCM 00058)):
Colony count (Saccharomyces cerevisiae ATCC 9763 (WDCM 00058)):
Recovery on test medium (Saccharomyces cerevisiae ATCC 9763 (WDCM 00058)): ≥ 50 %
Inoculum on reference medium (Aspergillus brasiliensis (formerly A. niger) ATCC 16404 (WDCM 00053)):
Colony count (Aspergillus brasiliensis (formerly A. niger) ATCC 16404 (WDCM 00053)):
Recovery on test medium (Aspergillus brasiliensis (formerly A. niger) ATCC 16404 (WDCM 00053)): ≥ 50 %
Inoculum on reference medium (Candida albicans ATCC 10231 (WDCM 00054)):
Colony count (Candida albicans ATCC 10231 (WDCM 00054)):
Recovery on test medium (Candida albicans ATCC 10231 (WDCM 00054)): ≥ 50 %
Inoculum on reference medium (Mucor racemus ATCC 42647 (WDCM00181)):
Colony count (Mucor racemus ATCC 42647 (WDCM00181)):
Recovery on test medium (Mucor racemus ATCC 42647 (WDCM00181)): ≥ 50 %
Growth (Escherichia coli ATCC 8739 (WDCM 00012)): no growth
Growth Escherichia coliATCC 25922 (WDCM 00013): no growth
Growth (Bacillus subtilis ATCC 6633 (WDCM 00003)): no growth
Incubation: 5 days at 25 ± 1 °C
A recovery rate of 50 % is equivalent to a productivity value of 0.5.
The indicated colony counts result from the sum of a triple determination.
Reference media: SABOURAUD dextrose agar
Appearance (colour): pink
pH-value (25 °C): 5.4 - 5.8
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Inoculum on reference medium (Saccharomyces cerevisiae ATCC 9763 (WDCM 00058)):
Colony count (Saccharomyces cerevisiae ATCC 9763 (WDCM 00058)):
Recovery on test medium (Saccharomyces cerevisiae ATCC 9763 (WDCM 00058)): ≥ 50 %
Inoculum on reference medium (Aspergillus brasiliensis (formerly A. niger) ATCC 16404 (WDCM 00053)):
Colony count (Aspergillus brasiliensis (formerly A. niger) ATCC 16404 (WDCM 00053)):
Recovery on test medium (Aspergillus brasiliensis (formerly A. niger) ATCC 16404 (WDCM 00053)): ≥ 50 %
Inoculum on reference medium (Candida albicans ATCC 10231 (WDCM 00054)):
Colony count (Candida albicans ATCC 10231 (WDCM 00054)):
Recovery on test medium (Candida albicans ATCC 10231 (WDCM 00054)): ≥ 50 %
Inoculum on reference medium (Mucor racemus ATCC 42647 (WDCM00181)):
Colony count (Mucor racemus ATCC 42647 (WDCM00181)):
Recovery on test medium (Mucor racemus ATCC 42647 (WDCM00181)): ≥ 50 %
Growth (Escherichia coli ATCC 8739 (WDCM 00012)): no growth
Growth Escherichia coliATCC 25922 (WDCM 00013): no growth
Growth (Bacillus subtilis ATCC 6633 (WDCM 00003)): no growth
Incubation: 5 days at 25 ± 1 °C
A recovery rate of 50 % is equivalent to a productivity value of 0.5.
The indicated colony counts result from the sum of a triple determination.
Reference media: SABOURAUD dextrose agar
Footnote
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
Legal Information
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
SAFETY INFORMATION
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
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