Description
DNA Isolation Kit, Plant Tissue, 75 reactions
Protocol and workflow optimized for Geno/Grinder and GenoLyte.
Reagents: Lysis Buffer, Binding Buffer B1, Wash Buffer PW1, Wash Buffer PW2, Elution Buffer, Proteinase K, RNase A
The Spex Genomic DNA Isolation Kit for plant tissue is optimized to work with the Genomax, Geno/Grinder, and GenoLyte, and allow for fast and seamless isolation of DNA from a wide variety of plant tissues.
The kit combines the use of chaotropic salts and detergents to disrupt plant tissues or cells permitting the selective binding of DNA to a silica column. Proteinase K and RNase A are then added to implement DNA isolation. An ethanol wash buffer is used to remove any additional elements or contamination from the silica column. The column is dried by centrifugation and DNA is eluted using the elution buffer.
Reagents | Lysis Buffer, Binding Buffer B1, Wash Buffer PW1, Wash Buffer PW2, Elution Buffer, Proteinase K, RNase A |
DNA Binding Columns and Collection Tubes | Silica-based columns capture DNA and allow for its purification |
Stainless Steel Balls | Five 3 mm 440C stainless steel balls |
Homogenization Tubes | Reinforced tubes designed for homogenization through rapid bead beating. Pre-loaded with five 3 mm 440C stainless steel balls |
Protocol | Protocol and workflow optimized for Genomax, Geno/Grinder, and GenoLyte |