Chloramphenicol, BioReagent, suitable for plant cell culture (25g)

SKU:
C1919-25G
$129.00

Description

Chloramphenicol

BioReagent, suitable for plant cell culture

Synonym: D-(−)-threo-2,2-Dichloro-N-[β-hydroxy-α-(hydroxymethyl)-β-(4-nitrophenyl)ethyl]acetamide, D-(−)-threo-2-Dichloroacetamido-1-(4-nitrophenyl)-1,3-propanediol, D-threo-2,2-Dichloro-N-[β-hydroxy-α-(hydroxymethyl)-4-nitrophenethyl]acetamide, Chloromycetin

  • CAS Number 56-75-7

     

  • Linear Formula Cl2CHCONHCH(CH2OH)CH(OH)C6H4NO2

     

  • Molecular Weight 323.13

     

  •  Beilstein/REAXYS Number 2225532

     

  •  EC Number 200-287-4

     

  •  MDL number MFCD00078159

     

  •  PubChem Substance ID 24892448

     

  •  NACRES NA.76

Properties

Related Categories A - K, Antibacterial, Antibiotics, Antibiotics A to Z, Antibiotics and Antimycotics,
Antibiotics by Application, Antibiotics by Mechanism of Action, Antiviral, Biochemicals and Reagents, Cell Culture, Cell Culture Antibiotics as Powder, Chemical Structure Class, Core Bioreagents, Genetic Marker Selection, Interferes with Protein Synthesis, Life Science Reagents for Cell Culture, Life Science Reagents for Transfection, List of Common Antibiotics A-F, Molecular Biology, Phenicoles, Plant Biotechnology, Plant Tissue Culture, Reagents and Supplements, Research Essentials, Spectrum of Activity
InChI Key WIIZWVCIJKGZOK-RKDXNWHRSA-N
product line BioReagent
form powder
application(s) cell culture | plant: suitable
impurities Endotoxin, tested
mp 148-150 °C (lit.)
solubility ethanol: 50 mg/mL
Featured Industry Agriculture
Mode of action protein synthesis | interferes
antibiotic activity spectrum Gram-negative bacteria
  Gram-positive bacteria
  mycobacteria
  mycoplasma
Gene Information human ... CYP1A2(1544)

General description

Chemical structure: phenicole

Packaging

5, 25, 100 g in glass bottle

Application

Chloramphenicol is a synthetic antibiotic, isolated from strains of Streptomyces venezuelae. It is often used for bacterial selection in molecular biology applications at 10-20 μg/mL and as a selection agent for transformed cells containing chloramphenicol reistance genes.

Biochem/physiol Actions

Mode of Action: Chloramphenicol inhibits bacterial protein synthesis by blocking the peptidyl transferase step by binding to the 50S ribosomal subunit and preventing attachment of aminoacyl tRNA to the ribosome. It also inhibits mitochondrial and chloroplast protein synthesis and ribosomal formation of (p)ppGpp, de-pressing rRNA transcription.

Mode of Resistance: Use of chloramphenicol acetyltransferase will acetylate the product and inactivate it.

Antimicrobial Spectrum: This is a broad spectrum antibiotic against gram-positive and gram-negative bacteria, and is used mainly for ophthalmic and veterinary purposes.

Caution

Stock solutions should be stored at 2-8°C and are stable at 37°C for 5 days. Aqueous solutions are neutral and stable over a wide pH range, with 50% hydrolysis occurring after 290 days. Use of a borax buffered solution reduces this number to 14%. Solutions should be protected from light as photochemical decomposition results in a yellowing of the solution. Heating aqueous solutions at 115°C for 30 minutes results in a 10% loss of chloramphenicol.

Preparation Note

Stock solutions can be prepared directly in the vial at any recommended concentration. A solution at 50 mg/mL in ethanol yields a clear, very faint, yellow solution. Degradation of chloramphenicol in aqueous solution is catalyzed by general acids and bases. This rate of degradation is independent of the ionic strength and pH.

Other Notes

Keep container tightly closed in a dry and well-ventilated place, Light sensitive. Storage class (TRGS 510): Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

Safety Information

Symbol 
GHS08
GHS08
Signal word 
Warning
Hazard statements 
H351
Precautionary statements 
P280
Personal Protective Equipment 
Eyeshields, full-face particle respirator type N100 (US), Gloves, respirator cartridge type N100 (US), type P1 (EN143) respirator filter, type P3 (EN 143) respirator cartridges
RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
AB6825000