2-Propanol, BioReagent, for molecular biology, 500ML

SKU:
I9516-500ML
  • $78.30

    • Description

    2-Propanol, BioReagent, for molecular biology, ≥99.5%, 500ML 

    Synonym(s):
    sec-Propyl alcohol, IPA, Isopropanol, Isopropyl alcohol
    Linear Formula:
    (CH3)2CHOH
    CAS Number:
    67-63-0
    Molecular Weight:
    60.10
    Beilstein:
    635639
    EC Number:
    200-661-7
    MDL number:
    MFCD00011674
    PubChem Substance ID:
    24896158
    NACRES:
    NA.52

    PROPERTIES

    grade

    for molecular biology

    Quality Level

    200

    vapor density

    2.1 (vs air)

    vapor pressure

    33 mmHg ( 20 °C)
    44 mmHg ( 25 °C)

    product line

    BioReagent

    Assay

    ≥99.5%

    form

    liquid

    autoignition temp.

    750 °F

    expl. lim.

    2.0-12.7 %, 93 °C

    technique(s)

    DNA extraction: suitable
    RNA extraction: suitable

    impurities

    ≤0.2% water
    water

    color

    colorless

    refractive index

    n20/D 1.377 (lit.)

    bp

    82 °C (lit.)

    mp

    −89.5 °C (lit.)

    solubility

    water: soluble (completely)

    density

    0.785 g/mL at 25 °C (lit.)

    suitability

    suitable for precipitation of nucleic acids

    storage temp.

    room temp

    SMILES string

    CC(C)O

    InChI

    1S/C3H8O/c1-3(2)4/h3-4H,1-2H3

    InChI key

    KFZMGEQAYNKOFK-UHFFFAOYSA-N

    General description

    Isopropanol is a clear, colorless, polar organic solvent commonly used in chemistry and molecular biology laboratories. It will dissolve a wide range of chemicals, and evaporates quickly. This product (2-propanol) is designated as molecular biology grade and is suitable for the precipitation of nucleic acids.

    Protocols for the use of isopropanol:

    • DNA precipitation from mammalian tissue, ethidium bromide extraction from DNA samples, and RNA isolation from cells and tissues.[1]
    • Protein crystallization.[2][3][4]
    • Isolate retinoids from biological matrices for HPLC and HPLC-MS analysis.[5][6]

    Application

    2-Propanol has been used:
    • in DNA and RNA extraction[7][8][9][10]
    • for determination of hydroxyproline levels(The Merck Index, 12th ed)
    • for checking cell viability by MTT assay[11]
    • in microarray hybridization[12]
    Suitable for use in DNA precipitation using standard protocols. Also suitable as a solvent for making solutions for molecular biology applications.

    SAFETY INFORMATION

    Pictograms

    GHS02,GHS07

    Signal Word

    Danger

    Hazard Statements

    H225 - H319 - H336

    Precautionary Statements

    P210 - P233 - P240 - P241 - P242 - P305 + P351 + P338

    Hazard Classifications

    Eye Irrit. 2 - Flam. Liq. 2 - STOT SE 3

    Target Organs

    Respiratory system

    Storage Class Code

    3 - Flammable liquids

    WGK

    WGK 1

    Flash Point(F)

    53.6 °F - closed cup

    Flash Point(C)

    12.0 °C - closed cup

    Personal Protective Equipment

    dust mask type N95 (US), Eyeshields, Gloves