Description
TWEEN 20 viscous liquid, 500ML
PROPERTIES
Quality Level
300
description
non-ionic
assay
≥40.0% (GC)
form
viscous liquid
mol wt
~1228
composition
lauric acid, ≥40% (balance primarily myristic, palmitic, and stearic acids)
application(s)
DNA extraction: suitable
RNA extraction: suitable
cell culture | mammalian: suitable
protein purification: suitable
impurities
≤3.0% water
refractive index
n20/D 1.468 (lit.)
CMC
0.06 mM (20-25°C)
acid number
≤2.2 mg/g
hydroxyl value
96‑108 mg/g
transition temp
cloud point 76 °C
density
1.095 g/mL at 25 °C (lit.)
HLB
16.7
suitability
complies for IR spectroscopy
InChI
1S/C26H50O10/c1-2-3-4-5-6-7-8-9-10-11-24(30)34-19-18-31-20-22(32-15-12-27)26-25(35-17-14-29)23(21-36-26)33-16-13-28/h22-23,25-29H,2-21H2,1H3
InChI key
HMFKFHLTUCJZJO-UHFFFAOYSA-N
DESCRIPTION
General description
Application
TWEEN 20 has been used as a blocking agent for membrane based immunoassays at a typical concentration of 0.05%. TWEEN 20 can be used for lysing mammalian cells at a concentration of 0.005 to 0.5%.
- washing buffer and blocking buffer in Western blotting
- blocking buffer in immunohistochemistry
- washing buffer in ELISA (enzyme linked immunosorbent assay)
- reaction mixture in PCR (polymerase chain reaction)
Packaging
Each kit contains 3 x 100ML samples, each sample from a uniquely manufactured lot.
Preparation Note
Legal Information
SAFETY INFORMATION
Storage Class Code
10 - Combustible liquids
WGK Germany
WGK 1
Flash Point(F)
527.0 °F - Pensky-Martens closed cup
Flash Point(C)
275 °C - Pensky-Martens closed cup
Personal Protective Equipment
dust mask type N95 (US),Eyeshields,Gloves