Description
TWEEN® 20
for molecular biology, viscous liquid
PROPERTIES
biological source
corn
plant (Palm)
plant (coconut)
wheat
Quality Level
300
grade
for molecular biology
description
non-ionic
Assay
>40% (GC)
form
viscous liquid
mol wt
~1228
~1228
composition
lauric acid, ≥40% (balance primarily myristic, palmitic, and stearic acids)
concentration
≥40.0% (GC)
impurities
RNAse, none detected
endonuclease, none detected
exonuclease, none detected
refractive index
n20/D 1.468 (lit.)
CMC
60 mg/L
solubility
water: 100 mg/mL, clear, colorless to yellow
density
1.095 g/mL at 25 °C (lit.)
suitability
suitable for molecular biology
foreign activity
Endonuclease-exonuclease and RNase, none detected
SMILES string
CCCCCCCCCCCC(=O)OCCOCC(C1C(C(CO1)OCCO)OCCO)OCCO
InChI
1S/C26H50O10/c1-2-3-4-5-6-7-8-9-10-11-24(30)34-19-18-31-20-22(32-15-12-27)26-25(35-17-14-29)23(21-36-26)33-16-13-28/h22-23,25-29H,2-21H2,1H3
InChI key
HMFKFHLTUCJZJO-UHFFFAOYSA-N
DESCRIPTION
General description
Surfactants, possessing both hydrophilic and hydrophobic components, fall into two main categories: ionic, which ionize in water (anionic, cationic, or ampholytic), and nonionic, which do not ionize. The discussed product is a nonionic surfactant with diverse applications such as emulsification, dispersion, solubilization, and detergent use. Additionally, it can serve as a solubilizer for membrane proteins.
Application
- As an emulsifying agent for the preparation of stable oil-in-water emulsions[2]
- In pre-extraction of membranes to remove peripheral proteins (used at 2% for extraction of membrane-bound proteins)[3]
- As a blocking agent for membrane based immunoassays at a typical concentration of 0.05%
- For lysing mammalian cells at a concentration of 0.005 to 0.5%
- Along with PBS in the dilution of antibodies in immunohistochemistry technique[4]
- For washing cells in FISH (Fluorescence in situ hybridization) technique[5]
Features and Benefits
- Highly versatile surfactant for your Life Science and Biochemical research
- Suitable for sensitive molecular biology research applications
- Tested for Endonuclease, exonuclease and Rnase′s
- Excellent solubilising, dispersing and emulsifying agent
Caution
Preparation Note
Legal Information
SAFETY INFORMATION
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
527.0 °F - Pensky-Martens closed cup
Flash Point(C)
275 °C - Pensky-Martens closed cup