Triton X-100 solution BioUltra, for molecular biology, in H2O

SKU:
93443-100ML
  • $139.00

    • Description

    Triton X-100 solution BioUltra, for molecular biology, ~10% in H2O

    Synonym(s):
    Triton™ X-100, 4-(1,1,3,3-Tetramethylbutyl)phenyl-polyethylene glycol solution, t-Octylphenoxypolyethoxyethanol, Polyethylene glycol tert-octylphenyl ether
    Linear Formula:
    t-Oct-C6H4-(OCH2CH2)xOH, x= 9-10
    CAS Number:
    9036-19-5
    Beilstein:
    2315025
    MDL number:
    MFCD00128254
    PubChem Substance ID:
    57653560

    PROPERTIES

    grade

    for molecular biology

    Quality Level

    200

    description

    non-ionic

    product line

    BioUltra

    form

    liquid

    mol wt

    micellar avg mol wt 80,000
    average mol wt 625

    concentration

    ~10% in H2O

    aggregation number

    100-155

    impurities

    DNases, none detected
    RNases, none detected
    insoluble matter, passes filter test
    phosphatases, none detected
    proteases, none detected

    refractive index

    n20/D 1.349±0.002

    pH

    6.5-8.5 (25 °C)

    CMC

    0.2-0.9 mM (20-25°C)
    0.23 mM

    transition temp

    cloud point 65 °C
    pour point ~7 °C

    density

    1.01 g/mL at 20 °C

    cation traces

    Al: ≤1 mg/kg
    Ba: ≤1 mg/kg
    Bi: ≤1 mg/kg
    Ca: ≤5 mg/kg
    Cd: ≤1 mg/kg
    Co: ≤1 mg/kg
    Cr: ≤1 mg/kg
    Cu: ≤1 mg/kg
    Fe: ≤1 mg/kg
    K: ≤100 mg/kg
    Li: ≤1 mg/kg
    Mg: ≤1 mg/kg
    Mn: ≤1 mg/kg
    Mo: ≤1 mg/kg
    Na: ≤100 mg/kg
    Ni: ≤1 mg/kg
    Pb: ≤1 mg/kg
    Sr: ≤1 mg/kg
    Zn: ≤1 mg/kg

    absorption

    cut-off at 310 nm

    HLB

    13.5

    SMILES string

    CC(C)(C)CC(C)(C)c1ccc(OCCOCCOCCOCCOCCOCCOCCO)cc1

    InChI

    1S/C28H50O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h6-9,29H,10-24H2,1-5H3

    InChI key

    HNLXNOZHXNSSPN-UHFFFAOYSA-N

    DESCRIPTION

    General description

    Triton™ X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection.[1][2]

    Application

    Triton™ X-100 has been used:
    • for permeabilization of cells before techniques like indirect immunofluorescence and flow cytometry[3][4]
    • As a component of lysis buffer used for homogenization of cells[3][4][5]
    • As a component of blocking buffer in immunofluorescence staining[6]
    • For the preparation of cells in double fluorescence staining[4]

    Biochem/physiol Actions

    Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.

    Features and Benefits

    • Suitable for Molecular Biology and Biochemical Research
    • Free from DNase, RNase, NICKase and protease
    • Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications.
    • High purity product for research applications
    • Tested for the presence of Anionic and Cationic traces

    Legal Information

    Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

    SAFETY INFORMATION

    Signal Word

    Danger

    Hazard Statements

    H315 - H318 - H410

    Precautionary Statements

    P273 - P280 - P302 + P352 - P305 + P351 + P338 + P310

    Hazard Classifications

    Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Skin Irrit. 2

    Storage Class Code

    10 - Combustible liquids

    WGK

    WGK 2

    Flash Point(C)

    251 °C - closed cup

    Personal Protective Equipment

    dust mask type N95 (US), Eyeshields, Gloves