MilliporeSigma
Sephadex G-50 BioReagent, for molecular biology, DNA grade, fine (25g)
- SKU:
- S5897-25G
Description
Sephadex® G-50
BioReagent, for molecular biology, DNA grade, fine
-
CAS Number 9004-54-0
-
MDL number MFCD00132771
Properties
grade | DNA grade |
- | for molecular biology |
product line | BioReagent |
form | beads |
swelling | 1 g swells to 9-11 mL |
bead diameter | 20-80 μm |
pore size | exclusion limit (20 bp dsDNA (rA)20) |
pH | 2 - 10 |
foreign activity | DNase, RNase, none detected |
storage temp. | room temp |
Description
Features and Benefits
• Excellent recovery
• Minimal sample dilution
• Quick and easy to use
General description
Sephadex® G-50, Fine is an often-cited gel filtration medium for desalting and buffer exchange of biomolecules > 30,000 MW. Sephadex® is prepared by crosslinking dextran with epichlorohydrin. Sephadex products differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. “Fine” indicates a small particle size known for excellent efficiency and short diffusion distances.
Packaging
25, 100 g in poly bottle
Application
Sephadex® G-50 is suitable for spin column purification of DNA (from contaminating small molecules) for use in hybridizations. It was used to purify PCR products prior to DNA sequencing to improve the sequencing quality.
Legal Information
Sephadex is a registered trademark of GE Healthcare