Sephadex G-50 BioReagent, for molecular biology, DNA grade, fine (25g)

SKU:
S5897-25G
  • $288.00

    • Description

    Sephadex® G-50

    BioReagent, for molecular biology, DNA grade, fine

    • CAS Number 9004-54-0

    •  MDL number MFCD00132771

    Properties

    grade DNA grade
     - for molecular biology
    product line BioReagent
    form beads
    swelling 1 g swells to 9-11 mL
    bead diameter 20-80 μm
    pore size exclusion limit (20 bp dsDNA (rA)20)
    pH 2 - 10
    foreign activity DNase, RNase, none detected
    storage temp. room temp

    Description

    Features and Benefits

    • Excellent recovery
    • Minimal sample dilution
    • Quick and easy to use

    General description

    Sephadex® G-50, Fine is an often-cited gel filtration medium for desalting and buffer exchange of biomolecules > 30,000 MW. Sephadex® is prepared by crosslinking dextran with epichlorohydrin. Sephadex products differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. “Fine” indicates a small particle size known for excellent efficiency and short diffusion distances.

    Packaging

    25, 100 g in poly bottle

    Application

    Sephadex® G-50 is suitable for spin column purification of DNA (from contaminating small molecules) for use in hybridizations. It was used to purify PCR products prior to DNA sequencing to improve the sequencing quality.

    Legal Information

    Sephadex is a registered trademark of GE Healthcare