MRC-5 pd25 Cell Line human,  05081101, fetal lung, 1 vial

SKU:
05081101-1VL
  • $810.59

    • Description

    MRC-5 pd25 Cell Line human, 

    05081101, fetal lung, 1 vial

    PROPERTIES

    biological source

    human lung

    growth mode

    Adherent

    karyotype

    2n=46, diploid

    morphology

    Fibroblast-like

    products

    Not specified

    receptors

    Not specified

    technique(s)

    cell culture | mammalian: suitable

    shipped in

    dry ice

    storage temp.

    −196°C

    Related Categories

    Mammalian Cell Lines

    DESCRIPTION

    Cell Line Origin

    Human foetal lung

    Cell Line Description

    Established from normal lung tissue of a 14 week old male foetus. The cells undergo between 60-70 population doublings before senescence. The virus susceptibility of this line to human viruses is similar to WI-38.

    Application

    Established from normal lung tissue of a 14 week old male foetus. The cells undergo between 60-70 population doublings before senescence. The virus susceptibility of this line to human viruses is similar to WI-38.

    DNA Profile

    STR-PCR Data: Amelogenin: X,Y
    CSF1PO: 11,12
    D13S317: 11,14
    D16S539: 9,11
    D5S818: 11,12
    D7S820: 10,11
    THO1: 8
    TPOX: 8
    vWA: 15

    Culture Medium

    EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

    Subculture Routine

    Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

    Other Notes

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