Imidazole buffer Solution BioUltra, 1 M in H2O (100mL)

SKU:
68268-100ML-F
  • $102.00

    • Description

    Imidazole buffer Solution

    BioUltra, 1 M in H2O

    Synonym: Glyoxaline solution, Imidazole solution

    • CAS Number 288-32-4

    • Empirical Formula (Hill Notation) C3H4N2

    • Molecular Weight 68.08

    •  Beilstein Registry Number 103853

    •  MDL number MFCD00005183

    Properties

    product line BioUltra
    concentration 1 M in H2O
    impurities DNases, none detected
     - RNases, none detected
    -  insoluble matter, passes filter test
    -  phosphatases, none detected
    -  proteases, none detected
    pH 9.5±0.5
    density 1.01 g/mL at 20 °C

    Description

    Application

    Imidazole can be used as a buffer in the pH range of 6.2-7.8 at 25°C. It is recommended as a component of a buffer for assay of horseradish peroxidase and as chelator for the binding of various divalent cations. Imidazole can be used for the elution of histidine containing proteins from divalent cation resins (Sigma P6611, HisSelect.-HC Nickel affinity gel) and can also be used in reverse staining of SDS-PAGE gels for detection of proteins.