Chloramphenicol, BioReagent, suitable for plant cell culture (25g)

SKU:
C1919-25G
  • $129.00

    • Description

    Chloramphenicol

    BioReagent, suitable for plant cell culture

    Synonym: D-(−)-threo-2,2-Dichloro-N-[β-hydroxy-α-(hydroxymethyl)-β-(4-nitrophenyl)ethyl]acetamide, D-(−)-threo-2-Dichloroacetamido-1-(4-nitrophenyl)-1,3-propanediol, D-threo-2,2-Dichloro-N-[β-hydroxy-α-(hydroxymethyl)-4-nitrophenethyl]acetamide, Chloromycetin

    • CAS Number 56-75-7

       

    • Linear Formula Cl2CHCONHCH(CH2OH)CH(OH)C6H4NO2

       

    • Molecular Weight 323.13

       

    •  Beilstein/REAXYS Number 2225532

       

    •  EC Number 200-287-4

       

    •  MDL number MFCD00078159

       

    •  PubChem Substance ID 24892448

       

    •  NACRES NA.76

    Properties

    Related Categories A - K, Antibacterial, Antibiotics, Antibiotics A to Z, Antibiotics and Antimycotics,
    Antibiotics by Application, Antibiotics by Mechanism of Action, Antiviral, Biochemicals and Reagents, Cell Culture, Cell Culture Antibiotics as Powder, Chemical Structure Class, Core Bioreagents, Genetic Marker Selection, Interferes with Protein Synthesis, Life Science Reagents for Cell Culture, Life Science Reagents for Transfection, List of Common Antibiotics A-F, Molecular Biology, Phenicoles, Plant Biotechnology, Plant Tissue Culture, Reagents and Supplements, Research Essentials, Spectrum of Activity
    InChI Key WIIZWVCIJKGZOK-RKDXNWHRSA-N
    product line BioReagent
    form powder
    application(s) cell culture | plant: suitable
    impurities Endotoxin, tested
    mp 148-150 °C (lit.)
    solubility ethanol: 50 mg/mL
    Featured Industry Agriculture
    Mode of action protein synthesis | interferes
    antibiotic activity spectrum Gram-negative bacteria
      Gram-positive bacteria
      mycobacteria
      mycoplasma
    Gene Information human ... CYP1A2(1544)

    General description

    Chemical structure: phenicole

    Packaging

    5, 25, 100 g in glass bottle

    Application

    Chloramphenicol is a synthetic antibiotic, isolated from strains of Streptomyces venezuelae. It is often used for bacterial selection in molecular biology applications at 10-20 μg/mL and as a selection agent for transformed cells containing chloramphenicol reistance genes.

    Biochem/physiol Actions

    Mode of Action: Chloramphenicol inhibits bacterial protein synthesis by blocking the peptidyl transferase step by binding to the 50S ribosomal subunit and preventing attachment of aminoacyl tRNA to the ribosome. It also inhibits mitochondrial and chloroplast protein synthesis and ribosomal formation of (p)ppGpp, de-pressing rRNA transcription.

    Mode of Resistance: Use of chloramphenicol acetyltransferase will acetylate the product and inactivate it.

    Antimicrobial Spectrum: This is a broad spectrum antibiotic against gram-positive and gram-negative bacteria, and is used mainly for ophthalmic and veterinary purposes.

    Caution

    Stock solutions should be stored at 2-8°C and are stable at 37°C for 5 days. Aqueous solutions are neutral and stable over a wide pH range, with 50% hydrolysis occurring after 290 days. Use of a borax buffered solution reduces this number to 14%. Solutions should be protected from light as photochemical decomposition results in a yellowing of the solution. Heating aqueous solutions at 115°C for 30 minutes results in a 10% loss of chloramphenicol.

    Preparation Note

    Stock solutions can be prepared directly in the vial at any recommended concentration. A solution at 50 mg/mL in ethanol yields a clear, very faint, yellow solution. Degradation of chloramphenicol in aqueous solution is catalyzed by general acids and bases. This rate of degradation is independent of the ionic strength and pH.

    Other Notes

    Keep container tightly closed in a dry and well-ventilated place, Light sensitive. Storage class (TRGS 510): Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

    Safety Information

    Symbol 
    GHS08
    GHS08
    Signal word 
    Warning
    Hazard statements 
    H351
    Precautionary statements 
    P280
    Personal Protective Equipment 
    Eyeshields, full-face particle respirator type N100 (US), Gloves, respirator cartridge type N100 (US), type P1 (EN143) respirator filter, type P3 (EN 143) respirator cartridges
    RIDADR 
    NONH for all modes of transport
    WGK Germany 
    3
    RTECS 
    AB6825000