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Description
Ascentis® Express 90 Å HILIC (5 μm) HPLC Columns
L × I.D. 25 cm × 3 mm, HPLC Column
About This Item
UNSPSC Code:
41115700
NACRES:
SB.52
Properties
product name
Ascentis® Express HILIC, 5 μm HPLC Column, 5 μm particle size, L × I.D. 25 cm × 3 mm
material
stainless steel column
Quality Level
100
agency
suitable for USP L3
product line
Ascentis®
feature
endcapped: no
manufacturer/tradename
Ascentis®
packaging
1 ea of
parameter
60 °C temp. range
600 bar max. pressure (9000 psi)
technique(s)
HPLC: suitable
LC/MS: suitable
L × I.D.
25 cm × 3 mm
surface area
90 m2/g
impurities
<5 ppm metals
matrix
Fused-Core particle platform
superficially porous particle
matrix active group
silica phase
particle size
5 μm
pore size
90 Å pore size
operating pH
1-8
application(s)
food and beverages
separation technique
hydrophilic interaction (HILIC)
normal phase
Description
General description
Ascentis Express 5 μm HILICis a high-speed, high-performance liquid chromatography column based on a 5-micron Fused-Core® particle design. The Fused-Core particle provides a thin porous shell of high-purity silica surrounding a solid silica core. This particle design exhibits very high column efficiency due to the shorter diffusion paths in the 0.6-micron thick porous shell around and an overall particle size of ~5-microns. The bare silica stationary phase of Ascentis Express 5 μm HILICprovides a column that can be used for traditional normal phase separations using non-polar, totally organic mobile phases or for aqueous normal phase chromatography with the typical reversed-phase mobile phases for hydrophilic interactive liquid chromatography (HILIC) of basic, acidic, or neutral compounds.
Column Characteristics
The Fused-Core particle has a surface area of ~ 100 m2/g and an average pore size of 90Å. The Fused-Core particles are 30% to 50% heavier than commercially available totally porous particles due to the density of the solid cores. Therefore, the effective surface area per column is similar to columns packed with totally porous particles having surface areas in the 160-200 m2/g range.
Column Characteristics
The Fused-Core particle has a surface area of ~ 100 m2/g and an average pore size of 90Å. The Fused-Core particles are 30% to 50% heavier than commercially available totally porous particles due to the density of the solid cores. Therefore, the effective surface area per column is similar to columns packed with totally porous particles having surface areas in the 160-200 m2/g range.
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Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Fused-Core is a registered trademark of Advanced Materials Technology, Inc.
Safety Information
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable