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2-Propanol BioReagent, for molecular biology, 4L

SKU:
I9516-4L
  • $302.00
  • Description

    2-Propanol

    BioReagent, for molecular biology, ≥99.5%, 4L

    Synonym: sec-Propyl alcohol, IPA, Isopropanol, Isopropyl alcohol

    • CAS Number 67-63-0

       
    • Linear Formula (CH3)2CHOH

       
    • Molecular Weight 60.10

       
    •  Beilstein/REAXYS Number 635639

       
    •  EC Number 200-661-7

       
    •  MDL number MFCD00011674

       
    •  PubChem Substance ID 24896158

       
    •  NACRES NA.52

    Properties

    Related Categories 2-Propanol (Isopropanol), Core Bioreagents, DNA & RNA Purification, Life Science Reagents for DNA/RNA Electrophoresis, Life Science Reagents for Protein Electrophoresis,
    Life Science Reagents for Protein Expression and Purification, Molecular Biology, Plant Biotechnology, Plant Molecular Biology, Plant Nucleic Acid Purification, Reagents, Research Essentials, Solvent by Type, Solvents
    Less...
    Quality Level 200
    grade for molecular biology
    vapor density 2.1 (vs air)
    vapor pressure 33 mmHg ( 20 °C)
     - 44 mmHg ( 25 °C)
    product line BioReagent
    assay ≥99.5%
    form liquid
    autoignition temp. 750 °F
    expl. lim. 2.0-12.7 %, 93 °C
    impurities ≤0.2% water
    water
    color colorless
    refractive index n20/D 1.377 (lit.)
    bp 82 °C (lit.)
    mp −89.5 °C (lit.)
    solubility water: soluble (completely)
    density 0.785 g/mL at 25 °C (lit.)
    suitability suitable for precipitation of nucleic acids
    storage temp. room temp
    SMILES string CC(C)O
    InChI 1S/C3H8O/c1-3(2)4/h3-4H,1-2H3
    InChI key KFZMGEQAYNKOFK-UHFFFAOYSA-N

    General description

    Isopropanol is a clear, colorless, polar organic solvent commonly used in chemistry and molecular biology laboratories. It will dissolve a wide range of chemicals, and evaporates quickly. This product (2-propanol) is designated as molecular biology grade and is suitable for the precipitation of nucleic acids.

    Protocols for the use of isopropanol:

    • DNA precipitation from mammalian tissue, ethidium bromide extraction from DNA samples, and RNA isolation from cells and tissues.
    • Protein crystallization.
    • Isolate retinoids from biological matrices for HPLC and HPLC-MS analysis.[1]

    Application

    2-Propanol has been used:
    • in DNA and RNA extraction[4][5][2]
    • for determination of hydroxyproline levels(The Merck Index, 12th ed)
    • for checking cell viability by MTT assay[6]
    • in microarray hybridization[7]

    Packaging

    25, 500 mL in glass bottle

    Safety Information

    Symbol 
    GHS02,GHS07
    Signal word 
    Danger
    Hazard statements 
    H225 - H319 - H336
    Precautionary statements 
    P210 - P233 - P240 - P241 - P242 - P305 + P351 + P338
    Target organs 
    Central nervous system
    Personal Protective Equipment 
    Eyeshields, Faceshields, Gloves, multi-purpose combination respirator cartridge (US), type ABEK (EN14387) respirator filter
    RIDADR 
    UN1219 - class 3 - PG 2 - Isopropanol
    WGK Germany 
    WGK 1
    RTECS 
    NT8050000
    Flash Point(F) 
    53.6 °F - closed cup
    Flash Point(C) 
    12.0 °C - closed cup