Description
2-Propanol
BioReagent, for molecular biology, ≥99.5%, 4L
Synonym: sec-Propyl alcohol, IPA, Isopropanol, Isopropyl alcohol
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CAS Number 67-63-0
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Linear Formula (CH3)2CHOH
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Molecular Weight 60.10
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Beilstein/REAXYS Number 635639
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EC Number 200-661-7
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MDL number MFCD00011674
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PubChem Substance ID 24896158
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NACRES NA.52
Properties
Related Categories | 2-Propanol (Isopropanol), Core Bioreagents, DNA & RNA Purification, Life Science Reagents for DNA/RNA Electrophoresis, Life Science Reagents for Protein Electrophoresis, Life Science Reagents for Protein Expression and Purification, Molecular Biology, Plant Biotechnology, Plant Molecular Biology, Plant Nucleic Acid Purification, Reagents, Research Essentials, Solvent by Type, Solvents Less... |
Quality Level | 200 |
grade | for molecular biology |
vapor density | 2.1 (vs air) |
vapor pressure | 33 mmHg ( 20 °C) |
- | 44 mmHg ( 25 °C) |
product line | BioReagent |
assay | ≥99.5% |
form | liquid |
autoignition temp. | 750 °F |
expl. lim. | 2.0-12.7 %, 93 °C |
impurities | ≤0.2% water water |
color | colorless |
refractive index | n20/D 1.377 (lit.) |
bp | 82 °C (lit.) |
mp | −89.5 °C (lit.) |
solubility | water: soluble (completely) |
density | 0.785 g/mL at 25 °C (lit.) |
suitability | suitable for precipitation of nucleic acids |
storage temp. | room temp |
SMILES string | CC(C)O |
InChI | 1S/C3H8O/c1-3(2)4/h3-4H,1-2H3 |
InChI key | KFZMGEQAYNKOFK-UHFFFAOYSA-N |
General description
Isopropanol is a clear, colorless, polar organic solvent commonly used in chemistry and molecular biology laboratories. It will dissolve a wide range of chemicals, and evaporates quickly. This product (2-propanol) is designated as molecular biology grade and is suitable for the precipitation of nucleic acids.
Protocols for the use of isopropanol:
• DNA precipitation from mammalian tissue, ethidium bromide extraction from DNA samples, and RNA isolation from cells and tissues.
• Protein crystallization.
• Isolate retinoids from biological matrices for HPLC and HPLC-MS analysis.[1]
Application
2-Propanol has been used:
• in DNA and RNA extraction[4][5][2]
• for determination of hydroxyproline levels(The Merck Index, 12th ed)
• for checking cell viability by MTT assay[6]
• in microarray hybridization[7]
Packaging
25, 500 mL in glass bottle