2-Mercaptoethanol, 25mL, for molecular biology, suitable for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration)

SKU:
M3148-25ML
  • $25.80

    • Description

    2-Mercaptoethanol, 25mL

    for molecular biology, suitable for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration)

    Synonym(s):
    β-Mercaptoethanol, 2-Hydroxyethylmercaptan, BME, Thioethylene glycol
    Linear Formula:
    HSCH2CH2OH
    CAS Number:
    60-24-2
    Molecular Weight:
    78.13
    Beilstein/REAXYS Number:
    773648
    EC Number:
    200-464-6
    MDL number:
    MFCD00004890
    PubChem Substance ID:
    24896800
    NACRES:
    NA.31

    PROPERTIES

    grade

    for molecular biology

    Quality Level

    200

    vapor density

    2.69 (vs air)

    vapor pressure

    1 mmHg ( 20 °C)

    product line

    BioReagent

    assay

    99% (GC/titration)

    form

    liquid

    expl. lim.

    18 %

    reaction suitability

    reagent type: reductant

    concentration

    14.3 M (pure liquid)

    technique(s)

    cell culture | mammalian: suitable
    electrophoresis: suitable

    refractive index

    n20/D 1.500 (lit.)

    pH

    4.5-6 (20 °C, 500 g/L)

    bp

    157 °C (lit.)

    solubility

    H2O: soluble 1 mL/mL

    density

    1.114 g/mL at 25 °C (lit.)

    foreign activity

    DNase, RNase, protease, none detected

    storage temp.

    room temp

    SMILES string

    OCCS

    InChI

    1S/C2H6OS/c3-1-2-4/h3-4H,1-2H2

    InChI key

    DGVVWUTYPXICAM-UHFFFAOYSA-N

    DESCRIPTION

    Application

    Reducing agent
    β-mercaptoethanol or 2-mercaptoethanol has been used:
    • as a supplement in Roswell Park Memorial Institute (RPMI)-1640 medium to culture DT40 cells (chicken B cell line)[1]
    • to lyse NuLi-1 (Normal Lung, University of Iowa-1) cells[2]
    • to trypsinize mouse embryonic stem cells[3]
    BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.

    SAFETY INFORMATION

    signalword

    Danger

    hcodes

    H301 + H331 - H310 - H315 - H317 - H318 - H361d - H373 - H410

    pcodes

    P273 - P280 - P301 + P310 - P302 + P352 + P310 - P304 + P340 + P311 - P305 + P351 + P338

    Hazard Classifications

    Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1A - STOT RE 2 Oral

    Storage Class

    6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials

    wgk_germany

    WGK 3

    flash_point_f

    154.4 °F - closed cup

    flash_point_c

    68 °C - closed cup

    ppe

    Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter