Description
Water, Nuclease-Free Water, for Molecular Biology, 10X50ML
Synonym(s):
DNase-Free Water, Not DEPC Treated, Nuclease-Free Water, Protease-Free Water, RNase-Free Water
Linear Formula:
H2O
CAS Number:
7732-18-5
Molecular Weight:
18.02
Beilstein:
2050024
EC Number:
231-791-2
MDL number:
MFCD00011332
PubChem Substance ID:
24901995
NACRES:
NA.31
PROPERTIES
vapor density
<1 (vs air)
Quality Level
200
vapor pressure
3 mmHg
sterility
0.1 μm filtered
form
liquid
technique(s)
PCR: suitable
electrophoresis: suitable
refractive index
n20/D 1.34 (lit.)
pH
5-7
bp
100 °C (lit.)
mp
0 °C (lit.)
density
1.000 g/mL at 3.98 °C (lit.)
foreign activity
DNase, RNase, Protease, free
SMILES string
O
InChI
1S/H2O/h1H2
InChI key
XLYOFNOQVPJJNP-UHFFFAOYSA-N
Application
Water has been used-
- in cDNA synthesis and PCR amplification[1][2]
- for the dilution of rumen DNA sample prior to qPCR assay[3]
- in PCR (polymerase chain reaction) and agarose gel electrophoresis[4]
- for the elution of RNA during RNA isolation[5]
Features and Benefits
- Nuclease-Free Water
- DNase-Free, RNase-Free, Protease- Free
- Not DEPC Treated
- 0.1 um Filtered
- Prepared from Double Distilled and Deionized Water
- Suitable for use in Molecular Applications
This product is 0.1um filtered. It is designated as a Molecular Biology Reagent and is suitable for molecular biology applications. This product has been analyzed for the absence of nucleases and proteases, and has undergone bioburden analysis. It has not been treated with diethyl pyrocarbonate (DEPC).
Preparation Note
This product is prepared from double distilled and deionized water
SAFETY INFORMATION
Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves