Description
Water, 1L
for molecular biology, sterile filtered
Synonym(s):
H2O
Linear Formula:
H2O
CAS Number:
7732-18-5
Molecular Weight:
18.02
Beilstein/REAXYS Number:
2050024
EC Number:
231-791-2
MDL number:
MFCD00011332
PubChem Substance ID:
57653665
NACRES:
NA.31
PROPERTIES
InChI key
XLYOFNOQVPJJNP-UHFFFAOYSA-N
General description
Molecular biology grade water is sterile filtered and ready to use, free from DNase, RNase, phosphatase, and protease contamination.
Application
Water, sterile filtered has been used:
- for cloning and plasmid construction.[1]
- for restriction digestion of PCR amplicons in polysaccharide A (PSA) restriction method.[2]
- in RNA isolation.[3][4][5]
- fluorescence in situ hybridization (FISH).[6]
- to dilute the samples of CVF (cervicovaginal fluid) and menstrual extracts for testing specificity through reverse transcriptase-polymerase chain reaction (RT-PCR) amplification of mRNA. [7]
- for DNA quantification using quantitative polymerase chain reaction (qPCR).[8]
SAFETY INFORMATION
Storage Class
12 - Non Combustible Liquids
wgk_germany
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
grade
for molecular biology
Quality Level
100
sterility
sterile-filtered
form
liquid
quality
sterile filtered
shelf life
limited shelf life, expiry date on the label
technique(s)
FISH: suitable
impurities
DNases, none detected
RNases, none detected
phosphatases, none detected
proteases, none detected
refractive index
n20/D 1.34 (lit.)
pH
5-7
conductivity
0.0-5 μS/cm at 25 °C
bp
100 °C (lit.)
mp
0 °C (lit.)
density
1.000 g/mL at 3.98 °C (lit.)
cation traces
Ca: <10 ppb
Mg: <10 ppb
SMILES string
O
InChI
1S/H2O/h1H2