Urea powder

SKU:
U5378-100G
  • $45.30

    • Description

    Urea Powder, BioReagent, for molecular biology, suitable for cell culture

    Synonym: Carbamide, Carbonyldiamide

    • CAS Number 57-13-6

       

    • Linear Formula NH2CONH2

       

    • Molecular Weight 60.06

       

    •  Beilstein/REAXYS Number 635724

       

    •  EC Number 200-315-5

       

    •  MDL number MFCD00008022

       

    •  PubChem Substance ID 24900656

       

    •  NACRES NA.31

    Properties

    Related Categories Biochemicals, Biochemicals and Reagents, Chaotropic Agents, Core Bioreagents, Denaturation,
    General Reagents, Life Science Reagents for Nucleic Acid Purification, Life Science Reagents for Protein Expression and Purification, Molecular Biology, Molecular Biology Reagents, Research Essentials
    Quality Level 200
    grade for molecular biology
    product line BioReagent
    assay ≥98%
    form powder
    application(s) cell culture | mammalian: suitable
    mp 132-135 °C (lit.)
    solubility H2O: soluble 480 mg/mL, clear, colorless (8M)
    density 1.335 g/mL at 25 °C (lit.)
    anion traces chloride (Cl-): ≤5 ppm
    cation traces Cu: ≤1 ppm
     - Fe: ≤5 ppm
     - Pb: ≤2 ppm
    absorption ≤0.06 at 260 at 6 M
     - ≤0.06 at 280 at 6 M
    foreign activity DNase, NICKase, RNase and protease, none detected
    SMILES string NC(N)=O
    InChI 1S/CH4N2O/c2-1(3)4/h(H4,2,3,4)
    InChI key XSQUKJJJFZCRTK-UHFFFAOYSA-N
    Gene Information human ... CA1(759), CA2(760)
    rat ... Ppm1a(24666)

    Description

    Application

    • Urea has been used in protein resuspension buffer and to denature proteins.[1]
    • It has been used for the isolation of proteins via urea-containing polyacrylamide gels.[2]
    • It has been used to study the conformation of unfolded proteins under different concentrations of urea and different pH.[3]

    Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.

    Packaging

    1 kg in poly bottle

    100, 500 g in poly bottle

    5 kg in poly drum

    Safety Information

    Personal Protective Equipment 
    Eyeshields, Gloves, type N95 (US)
    RIDADR 
    NONH for all modes of transport
    WGK Germany 
    WGK 1
    RTECS 
    YR6250000
    Flash Point(F) 
    Not applicable
    Flash Point(C) 
    Not applicable