Trizma hydrochloride solution, 1L

SKU:
T1819-1L
  • $90.90

    • Description

    Trizma® hydrochloride solution

    pH 7.0, BioPerformance Certified, 1 M, suitable for cell culture

    Synonym(s):

    Tris hydrochloride solution

    About This Item

    UNSPSC Code:
    12161700
    NACRES:
    NA.25

    Properties

    grade

    BioPerformance Certified
    for molecular biology

    form

    liquid

    concentration

    1 M

    technique(s)

    cell culture | mammalian: suitable

    impurities

    DNase, RNase, Protease, none detected
    bioburden, tested
    endotoxin, tested
    ≤5 ppm Heavy metals (as Pb)

    pH

    7.0

    useful pH range

    7.0-9.0

    absorption

    ≤0.05 at 290 at 40%

    General description

    Tris(hydroxymethyl)aminomethane (Tris) is a standard buffer in biological sciences and has a pH buffering range between 7.0 - 9.0.[1] It is applicable in electrophoretic studies and capillary electrochromatography.[2]
    A series of Pre-mixed solutions of TRIZMA Base and TRIZMA HCl to provide commonly used pH values for Tris buffers. No mixing or pH adjustment necessary. Guaranteed accuracy ± 0.1 pH units.

    Application

    Trizma® hydrochloride solution has been used:
    • as a buffer in reverse transcription and DNase I treatment reactions for RNA isolated from Toxoplasma gondii[3]
    • in the preparation of tris- ethylenediaminetetraacetic acid (EDTA) (TE) buffer for bacterial pellet resuspension for plasmid isolation studies[4]
    • for polylactic acid nanoparticle resuspension, storage, and pH-based stability studies[5]

    Legal Information

    Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

    Safety Information

    Storage Class

    10 - Combustible liquids

    wgk_germany

    WGK 2

    flash_point_f

    Not applicable

    flash_point_c

    Not applicable

    ppe

    Eyeshields, Gloves, type ABEK (EN14387) respirator filter