Description
SIGMAFAST™ Protease Inhibitor Tablets
For General Use
Properties
| storage temp. | 2-8°C |
Description
Components
AEBSF, 2 mM
Aprotinin, 0.3 μM
Bestatin, 130 μM
EDTA, 1 mM
E-64, 14 μM
Leupeptin, 1 μM
Other Notes
Tableted formulation containing water-soluble protease inhibitors with broad specificity for the inhibition of serine, cysteine, and metalloproteases. Contains 4-(2-aminoethyl)benzenesulfonyl fluoride (AEBSF), E-64, bestatin, leupeptin, aprotinin, and EDTA (sodium salt).
Quantity
One tablet makes 100 mL of cocktail. One mL is recommended for the inhibition of proteases equivalent to 1 mg of USP pancreatin. One tablet is recommended for the inhibition of proteases present in a maximum of 20 g of cell extract.
Specificity
Inhibits serine, cysteine, aspartic, and metalloproteases.
Physical form
Tablet that is water soluble.
Application
Optimized and tested for general use.
SIGMAFAST™ Protease Inhibitor Tablets has been used to collect tracheae in combination with ice-cold radioimmunoprecipitation assay buffer in a study to determine the tracheal basal cell fate. It has been used to homogenize rat lung tissues for western blot analysis.
Legal Information
SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC
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