Roche Isopropyl-beta-D-thiogalactoside (IPTG) (1g)

SKU:
10724815001
  • $88.60

    • Description

    IPTG-RO ROCHE

    Isopropyl-β-D-thiogalactoside

    Synonym: IPTG, Isopropyl β-D-1-thiogalactopyranoside, Isopropyl β-D-thiogalactoside, iptg

    • CAS Number 367-93-1

    • Empirical Formula (Hill Notation) C9H18O5S

    • Molecular Weight 238.30

    •  Beilstein Registry Number 4631

    •  MDL number MFCD00063273

    Properties

    mol wt mol wt 238.3
    packaging pkg of 1 g (10724815001)
    mfr. no. Roche
    shipped in wet ice
    storage temp. 2-8°C

    Description

    Application

    IPTG is commonly used in cloning procedures that require induction of β-galactosidase activity. It is used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lacoperon in Escherichia coli. IPTG functions by binding to the lacI repressor and altering its conformation, which prevents the repression of the β-galactosidase coding gene lacZ.

    Isopropyl-β-D-thiogalactoside (IPTG) is commonly used in cloning procedures that require induction of β-galactosidase activity. It is used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli. It has been used for the expression of recombinant genes in E. coli.[1][2]

    Other Notes

    Non-metabolizable galactose analog.

    For life science research only. Not for use in diagnostic procedures.

    The amount of labeled DNA depends on:
    • amount of template DNA
    • purity of template DNA
    • conformation of template DNA
    • average size of labeled fragment

    Preparation Note

    Sample Material
    10 ng to 3μg template DNA, linearized and at least 100 to 200bp long

    Working concentration: Final concentration should be 0.2 mM.
    Storage conditions (working solution): Stock solutions of the product in water (e.g., 1 M IPTG) stored at -15 to -25 °C, are stable up to six months.

    General description

    Isopropyl-β-D-thiogalactoside (IPTG) is a chemical analog of galactose that cannot be cleaved by β-galactosidase. IPTG functions by binding to the lacI repressor and altering its conformation, which prevents the repression of the β-galactosidase coding gene lacZ.[3]

    Specifications

    Assay Time: 50minutes
    Specific Activity: The described standard assay will results in a specific activity of 1.8 x 109 dpm/μg, corresponding to 65% incorporation with different substrate DNAs in 30minutes. When varying the ratio of template DNA to labeled dNTP, similar incorporation rates, but different levels of specific activity of the labeled probe are obtained.