Description
Recombinant Tri a 14 (Triticum aestivum allergen 1)
We are proud to introduce a new comprehensive range of purified food allergen protein standards manufactured by Indoor Biotechnologies. Indoor Biotechnologies’ allergens are purified by affinity chromatography and/or HPLC. Protein identity and amino acid composition are verified by InBio® using MS (mass spectrometry) and amino acid analysis. Immune reactivity is validated by monoclonal antibody binding. Purified allergens are also validated for IgE antibody binding. Allergens are purified and validated via the processes described above under an ISO 9001:2015 certified Quality Management System, providing for high quality and consistent allergen proteins.
The proteins are either isolated from a natural source (product codes NA) or recombinant (RE, RP or RPI). Some proteins are also available in biotinylated form (BI). LoToxTM proteins (LTN or LTR) have very low endotoxin levels (<0.03EU/μg protein)
Indoor Biotechnologies is headquartered in Charlottesville, VA and has more than 25 years of experience in
the production of highly purified proteins and immunoassays for research and diagnostics. The company has
specialized in environmental allergen detection systems as well as purified natural and genetically engineered
allergens for research and molecular diagnostics. Indoor Biotechnologies is internationally recognized for research
on protein structure, function and immune recognition.
PROPERTIES
grade
analytical standard
Assay
>95%
mol wt
10 kDa
packaging
pkg of 250 μg
manufacturer/tradename
InBio
technique(s)
HPLC: suitable
LC/MS: suitable
application(s)
food and beverages
format
neat
storage temp.
−20°C
Source: E. coli
Formulation: Preservative and carrier-free in 20mM Tris, 0.3M NaCl, 0.2M L-arginine pH 8.0. Filtered through 0.22μm filter.
Concentration: See product insert.
Purification: Purified from E. coli culture by multi-step HPLC chromatography. Purity >95% by SDS-PAGE
Note:
(1)rTri a 14 has a C-terminal 6xHis-tag.
(2) Avoid repeated Freeze/Thaw cycles. It is recommended to aliquot the product upon first thaw and freeze in working aliquot volumes.
Disclaimer
SAFETY INFORMATION
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1