Quant X - Fungal (QF-003)

Quant X - Fungal, (QF-003)

Quantify TY&M in a 96 well plate.

Total Yeast & Mold (Quant 10^4, 10^3, 10^2)

QuantX  is a quantitative microbiological diagnostic assay that will enumerate different bacterial or fungal indicator classes. This array is designed to meet the needs of state and country regulations where quantitation of Total Aerobic Bacteria (TAB), Bile-Tolerant Gram Negative (BTGN), Total Enterobacteriaceae (ENT; including Total Coliform) and Total Yeast and Mold (TYM) is required. The assay will establish linearity and quantitation of signal through the use of synthetic DNA fragments included in PCR and hybridization. The synthetic DNA fragment is designed to be similar in length and sequence structure to the target sequence, thus ensuring similar amplification during PCR. The fragment has multiple nucleotide mismatches purposely designed into the sequence, to ensure no cross-reactivity between the target sequence, and the synthetic DNA fragment. The assay will provide results as CFU/gram values, with standard deviations below those reported for traditional plate cultures.

With its proprietary technologies, PathogenDx designs and develops nucleic acid test kits and automation for the detection and speciation of a few, up to several dozen microbial or viral pathogens as a single test panel. The kits and associated imager (SensospotTM) are sold to regulated testing labs in Cannabis, Food, IVD and Environmental Screening markets. Although the pathogen test content varies based on the market application, the technology has as its core a type of low cost DNA microarray, mass produced as a 96 well SBS-formatted microplate, delivering up to 400 oligonucleotide hybridization tests in parallel, per well.  In each kit, the microarray plate is provided with all reagents needed for automated or manual sample preparation (96 samples) then multiplex asymmetric PCR or RT-PCR (96 samples) which then  generates amplicon products which are then analyzed (up to 96 samples in parallel) on a single SBS-Format plate. Subsequent to room temperature hybridization and washing, by hand or with an ordinary off-the-shelf fluid handling robot, the resulting microarray test is imaged on a dedicated 96 well plate imager (SensospotTM). All 96 array images are then analyzed via simplified, hands-free, autonomous software (AuguryTM) that is provided as a no-cost license with the kits. The software is configured to run locally, or as part of a cloud-based testing system, which is accessed over the internet on a secure Portal. The Portal, also provided as a no-cost license, enables secure data storage and sharing and technical support from PathogenDx staff.