Proteinase K from Tritirachium album, lyophilized powder, BioUltra, for molecular biology

SKU:
P2308-100MG
  • $181.00

    • Description

    Proteinase K from Tritirachium album, lyophilized powder, BioUltra, ≥30 units/mg protein, for molecular biology

    Synonym(s):
    Endopeptidase K
    CAS Number:
    39450-01-6
    Enzyme Commission number:
    3.4.21.64 (BRENDA, IUBMB)
    EC Number:
    254-457-8
    MDL number:
    MFCD00132129
    eCl@ss:
    32160410
    NACRES:
    NA.54

    grade

    for molecular biology

    Quality Level

    400

    product line

    BioUltra

    form

    lyophilized powder

    specific activity

    ≥30 units/mg protein

    mol wt

    28.93 kDa

    composition

    Protein, ≥90% biuret

    impurities

    ≤1 ppm DNA

    foreign activity

    DNAse, Nickase and RNAse, none detected

    shipped in

    wet ice

    storage temp.

    −20°C

    Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA.
    Removes endotoxins that bind to cationic proteins such as lysozyme and ribonuclease A.
    Reported useful for the isolation of hepatic, yeast, and mung bean mitochondria
    Determination of enzyme localization on membranes
    Treatment of paraffin embedded tissue sections to expose antigen binding sites for antibody labeling.
    Digestion of proteins from brain tissue samples for prions in Transmissible Spongiform Encephalopathies (TSE) research.

    5 mg in glass bottle
    10, 25, 100, 500 mg in poly bottle
    1 g in poly bottle

    Biochem/physiol Actions

    Proteinase K is a stable and highly reactive serine protease. Evidence from crystal and molecular structure studies indicates the enzyme belongs to the subtilisin family with an active-site catalytic triad (Asp39-His69-Ser224). It is stable in a broad range of environments: pH, buffer salts, detergents (SDS), and temperature. In the presence of 0.1-0.5% SDS, proteinase K retains activity and will digest a variety of proteins and nucleases in DNA preparations without compromising the integrity of the isolated DNA.

    Unit Definition

    One unit will hydrolyze urea-denatured hemoglobin to produce color equivalent to 1.0 μmole of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).

    Pictograms

    GHS08

    Signal Word

    Danger

    Hazard Statements

    H315 - H319 - H334

    Precautionary Statements

    P261 - P264 - P280 - P284 - P302 + P352 - P305 + P351 + P338

    Hazard Classifications

    Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2

    Storage Class Code

    11 - Combustible Solids

    WGK

    WGK 1

    Flash Point(F)

    Not applicable

    Flash Point(C)

    Not applicable

    Personal Protective Equipment

    dust mask type N95 (US), Eyeshields, Gloves