PLTGold Human Platelet Lysate, 500mL

SKU:
SCM152
  • $2,130.00

    • Description

    PLTGold Human Platelet Lysate, 500mL

    Heparin-free Human Platelet Lysate (hPL) for Mesenchymal Stem Cell Culture. 500ml

    DATA SHEET

    PLTGold Human Platelet Lysate is a Xeno-Free cell culture supplement that is a superior alternative to fetal bovine serum (FBS) for use in human mesenchymal stem cell cultures. PLTGold is an unfractionated product derived from human platelets that does not require the addition of heparin. It remains clot-free with performance equivalent to the original PLTMax platelet lysate. Optimal growth of MSCs can be achieved with 5% PLTGold. PLTGold been used to effectively to grow mesenchymal stem cells derived from other species including mouse, rabbit and porcine.

    PLTGold Human Platelet Lysate is derived from normal human donor platelets collected at U.S. blood centers. Multiple donors units are pooled in large batch sizes and manufactured to produce a consistent product. Human Platelet Lysate is for research use only (RUO) and not intended for human or animal diagnostic or therapeutic use.

    Application

    Research Category
    Stem Cell Research
    Research Sub Category
    Mesenchymal Stem Cells
    Heparin-free Human Platelet Lysate (hPL) for Mesenchymal Stem Cell Culture. 500ml.
    Instructions for Use

    1.Cell seeding should be performed following the general guidelines for the specific cell type. For Mesenchymal Stem Cells (MSCs), cells are typically plated at approximately 2x10^3 – 5x10^3 cells per cm2.
    2. For MSCs, PLTGold can be used at 5% vol/vol in a typical cell culture medium such as DMEM (Sigma D5671) or α-MEM (Sigma M8042). If the basic media doesn’t contain Glutamine, a source of L-Glutamine (Sigma G7513) will need to be added to the media at a final concentration of 2mM. For other types of cells, the concentration of PLTGold will need to be titrated according to the application (a titration from 2% vol/vol to 10% vol/vol is recommended to establish the percentage of PLTGold needed for the cell type to use).
    3. Do not allow primary MSC confluence to exceed 70-80%.
    All human derived materials are obtained from government inspected facilities and are of US origin. Each donor unit was approved for human use and has been tested for infectous diseases including HIV-1, HIV-2, HIV-NAT, HCV, HBsAg, and RPR for Syphilis.

    Each lot is extensively tested for the following parameters:
    Sterility Testing: Negative for growth
    pH: 6.8-7.8
    Total protein: 4.0-6.5 g/dl
    Osmolality: 250-330 mOsm/kg
    Endotoxin USP <8>: <10 EU/mL
    Mycoplasma: Not detected
    Cell Growth: Pass

    Storage and Stability

    Store at -80°C upon receipt. Thaw at room temperature or in a water bath. After thawing, aliquot and store at -20°C or colder. Long term storage at -80°C is recommended. Multiple freeze/thaw cycles are not recommended.

    Legal Information

    PLTGold is a registered trademark of Mill Creek Life Sciences, LLC

    Disclaimer

    Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

    SAFETY INFORMATION

    Storage Class Code

    10 - Combustible liquids

    WGK

    nwg

    Flash Point(F)

    Not applicable

    Flash Point(C)

    Not applicable