Description
Phosphate Buffered Saline 10× PBS for Western blots and IP
Synonym(s):
Phosphate buffered saline, PBS
MDL number:
MFCD00131855
eCl@ss:
32129211
NACRES:
NA.25
PROPERTIES
Quality Level
200
grade
for molecular biology
sterility
0.2 μm filtered
form
liquid
pH
7.2-7.6(1x solution)
storage temp.
room temp
DESCRIPTION
General description
Phosphate Buffered Saline (PBS) is routinely used as a wash buffer in Western blot and immunoprecipitation (IP) procedures, as well as during sample preparation of cell lysates for use in general molecular biology applications.
Application
Suitable for use in wash buffers for Western blots and IP, as well as cell lysate sample preparation.
Components
10x PBS contains:
- 30mM sodium phosphate
- 1.5 M sodium chloride
- 10.5 mM potassium phosphate
Preparation Note
Dilute 10x PBS to a 1x working solution using molecular biology grade water.
SAFETY INFORMATION
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable