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Peptone Water, phosphate-buffered, 500G

SKU:
77187-500G
  • $108.00

    • Description

    Peptone Water, phosphate-buffered, 500G

    Suitable for microbiology, NutriSelect Plus

    Synonym(s):
    BPW, Buffered Peptone Water, Tryptone Phosphate Water
    NACRES:
    NA.85

    Quality Level

    200

    agency

    according to GB 4789.30-2016
    according to GB 4789.40-2016
    according to ISO 21528-1:2017
    according to ISO 22964:2017

    sterility

    non-sterile

    product line

    BioChemika

    form

    powder

    shelf life

    limited shelf life, expiry date on the label

    composition

    disodium hydrogen phosphate, 3.5 g/L
    peptone, 10 g/L
    potassium dihydrogen phosphate, 1.5 g/L
    sodium chloride, 5 g/L

    manufacturer/tradename

    NutriSelect® Plus

    technique(s)

    microbe id | specific enzyme detection: suitable
    microbiological culture: suitable

    final pH

    7.2±0.2 (25 °C)

    application(s)

    environmental
    food and beverages
    pathogen testing
    sample preparation
    microbiology

    suitability

    Providencia spp.
    nonselective for Citrobacter spp.
    nonselective for Cronobacter spp.
    nonselective for Escherichia coli
    nonselective for Klebsiella spp.
    nonselective for Proteus spp.
    nonselective for Pseudomonas spp.
    nonselective for Salmonella spp.
    nonselective for Shigella spp.
    nonselective for Staphylococcus spp.
    nonselective for Streptococcus spp.
    nonselective for Yersinia spp.
    nonselective for bacteria (General Media)
    nonselective for coliforms
    nonselective for enterobacteriaceae

    Buffered peptone water being suitable as first diluent is helpful since the same homogenate can be used for detection of other microorganisms. Pre-enrichment of the culture media is performed using buffered peptone water to increase target concentration and improve sensitivity. It is also useful for the non-selective pre-enrichment of media for bacteria, in particular pathogenic members of the Enterobacteriaceae (sub-lethal damaged), from food and other material.

    Application

    Buffered peptone water may be used to homogenize poultry samples. It may also be used in microbial culture of Staphylococcus aureus and followed by GC-MS analysis to determine the chemical composition of galangal extraxt and HPLC analysis to isolate compounds of Indian gooseberry extract.

    Preparation Note

    Dissolve 20 g in 1 litre distilled water. Sterilize by autoclaving at 121°C for 15 minutes.

    Other Notes

    Studies with salmonellae

    Footnote

    We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
    The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

    Legal Information

    GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
    NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

    SAFETY INFORMATION

    Storage Class Code

    13 - Non Combustible Solids

    WGK

    WGK 3

    Flash Point(F)

    Not applicable

    Flash Point(C)

    Not applicable

    Personal Protective Equipment

    dust mask type N95 (US), Eyeshields, Gloves