PCR 100 bp Low Ladder for electrophoresis of PCR fragments

SKU:
P1473-1VL
  • $139.00

    • Description

    PCR 100 bp Low Ladder for electrophoresis of PCR fragments

    Synonym(s):
    100bp maker, 100bp marker for agarose gel electrophoresis, 100pb ladder for agarose gel electrophoresis, DNA ladder, DNA marker, gel ladder, gel marker

    PROPERTIES

    grade

    for molecular biology

    Quality Level

    200

    form

    liquid

    usage

    75 uses

    suitability

    suitable for electrophoresis (DNA)

    storage temp.

    −20°C

    DESCRIPTION

    General description

    Sigma′s PCR 100 bp Low Marker contains 10 dsDNA fragments from 100 – 1,000 bases, evenly spaced in 100 bp repeats. Two ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose gel.
    The PCR 100 bp low ladder has been specially designed for the size determination of polymerase chain reaction (PCR) generated DNA fragments.

    Application

    PCR 100 bp Low Ladder has been used:
    • in agarose gel electrophoresis as a standard DNA marker to obtain DNA fragments of about 240 bp for polymerase chain reaction (PCR)-denaturing gradient gel electrophoresis (DGGE) analysis
    • to visualize DNA from enzyme digested PCR mixture
    • to estimate the molecular weight of the PCR product fragments

    Components

    Sigma′s PCR 100 bp Low Marker is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

    Other Notes

    Contains 10 bands, 100–1,000 bp in exact 100 bp spaced (ladder) recombinant repeats.
    For optimal resolution, the recommended agarose gel concentration is 2.0%.

    SAFETY INFORMATION

    Storage Class Code

    12 - Non Combustible Liquids

    WGK

    WGK 3

    Flash Point(F)

    Not applicable

    Flash Point(C)

    Not applicable

    Personal Protective Equipment

    dust mask type N95 (US), Eyeshields, Gloves