Mueller Hinton Agar

SKU:
70191-500G
  • $125.00

    • Description

    Mueller Hinton Agar

    suitable for microbiology, NutriSelect® Plus

    PROPERTIES

    sterility

    non-sterile

    Quality Level

    200

    product line

    BioChemika

    form

    powder

    shelf life

    limited shelf life, expiry date on the label

    composition

    agar, 17.0 g/L
    beef infusion solids, 2.0 g/L
    casein hydrolysate, 17.5 g/L
    starch, 1.5 g/L

    manufacturer/tradename

    NutriSelect® Plus

    technique(s)

    microbe id | susceptibility testing: suitable
    microbiological culture: suitable

    final pH

    7.3±0.2 (25 °C)

    application(s)

    clinical testing
    food and beverages
    microbiology

    storage temp.

    10-30°C

    suitability

    nonselective for Neisseria spp.
    nonselective for bacteria (General Media)

    General description

    Mueller Hinton Agar is specially designed for antibiotic susceptibility testing using the disc diffusion method (Kirby Bauer method). It has been recommended by the CLSI as the ideal medium for antibiotic susceptibility testing for the following reasons:

    • It shows good reproducibility from batch to batch
    • It is low in certain inhibitors like trimethoprim, sulfonamide and tetracycline
    • It supports the growth of both fastidious and non-fastidious bacterial pathogens
    • The starch in the media absorbs toxins thus preventing them from interfering with the antibiotics
    • It is a loose agar and therefore facilitates better diffusion of antibiotics
    Mueller Hinton Agar has been used:
    • In culturing Escherichia coli ATCC™ 25922 strain for disc diffusion studies
    • In disk diffusion assay of fusidic acid against Staphylococcus aureus, Bacillus cereus, Pseudomonas aeruginosa and Escherichia coli
    • In agar diffusion assay of drug-loaded nano-hydroxyapatite formulations against Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli

    Application

    Mueller Hinton Agar is a solid medium originally designed for the isolation of pathogenic Neisseria species, now widely used for antibiotic susceptibility testing (including sulfonamides) of aerobic and facultatively anaerobic bacteria isolated from clinical specimens.

    Preparation Note

    Suspend 38 g in 1 litre of distilled water, bring to the boil to dissolve the medium completely and sterilize by autoclaving at 121°C for 15 minutes.

    Other Notes

    Enhancement of cefotoxamine and other cephalosporins against Enterococcus faecalis by blood supplemented Mueller-Hinton agar[1][2]

    Footnote

    We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
    The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

    Legal Information

    GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
    NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

    SAFETY INFORMATION

    Storage Class

    11 - Combustible Solids

    wgk_germany

    WGK 3

    flash_point_f

    Not applicable

    flash_point_c

    Not applicable

    ppe

    Eyeshields, Gloves, type N95 (US)