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Microbial DNA standard from Bacillus subtilis Suitable for PCR, Sequencing and NGS, 10 ng/uL (0.3ug)

SKU:
MBD0018-0.3UG
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  • Description

    MBD0018 Sigma-Aldrich

    Microbial DNA standard from Bacillus subtilis

     

    Description

    General description

    Microbial DNA standard from Bacillus subtilis (B. subtilis) is a genomic DNA microbial standard that can serve as a standard for benchmarking the performance, along the workflow of microbiomics or meta-genomics analyses. It can also be used as a tool to increase reproducibility and allow comparison of results obtained by different labs. Standardization of sample analysis is currently needed in microbiome genomics research workflow. Lack of standardization can lead to biases and errors in common processes during sample preparation and analysis such as sample amplification, sequencing and bioinformatics analyses. Bacillus subtilis is a gram positive,[1] aerobic bacterium, rod-shaped, endospore-forming, catalase-positive. It is traditionally considered as a soil microorganism. Bacillus subtilis is genetically well characterized and serves as a model organism for important gram-positive pathogens such as Bacillus anthracisStaphylococcus aureus[2] etc. B. subtilis possess an extreme adaptation ability to various environmental stress conditions by modifying its motility, uptake of exogenous DNA, biofilm formation and sporulation. Recent studies have identified and characterized B. subtilis isolates from human gastrointestinal track suggesting B. subtilis as a gut commensal. Moreover, B. subtilis presence in the gut was found to correlate with probiotic effects such as anti-diarrhea effect, anti-microbial effect, immune-stimulatory effect, competition with pathogens, normal flora growth stimulation and prevention of intestinal inflammation. B. subtilis beneficial effect on gut inflammation may also suggest it as potential probiotic treatment for inflammatory bowel diseases (IBD),[3] including Crohn′s and ulcerative colitis. In addition, it was demonstrated that a peptide produced by B. subtilis possess antiviral effect against influenza virus.

    Application

    Suitable for Quantitative standard for PCR, Sequencing and NGS

    Features and Benefits

    • Individual microbial standard for microbiomics and meta-genomics workflow
    • Suitable standard for PCR, sequencing and NGS
    • Improve Bioinformatics analyses
    • Increases reproducibility
    • Compare results lab to lab

    Physical form

    Liquid - The genomic DNA is provided at >=10 ng/μL concentration in TE buffer pH 8.0

    Other Notes

    It is recommended to avoid freeze thaw cycles of this product.

     

    Properties

    Related Categories Microbial DNA Standard, Microbiome Standards, Molecular Biology, Proteomics
    form   liquid
    concentration   10 ng/μL
    shipped in   ambient
    storage temp.   −20°C