Methylene Blue solution for microscopy, concentrated, aqueous solution, 250mL

SKU:
03978-250ML
  • $53.70

    • Description

    Methylene Blue solution

    for microscopy, concentrate according to Ehrlich, concentrated, aqueous solution

    Synonym(s):
    Methylene blue stain, Ehrlich’s reagent III, Loeffler’s Methylene Blue
    Empirical Formula (Hill Notation):
    C16H18ClN3S
    CAS Number:
    61-73-4
    Molecular Weight:
    319.85
    Beilstein/REAXYS Number:
    3641570
    MDL number:
    MFCD00012111
    PubChem Substance ID:
    329747853
    NACRES:
    NA.47

    PROPERTIES

    grade

    for microscopy

    Quality Level

    100

    form

    liquid

    quality

    concentrated, aqueous solution

    shelf life

    ~3 yr

    concentration

    1.5% (1.5 g/ 100 mL)

    density

    1.0 g/mL at 20 °C

    εmax

    9.4 at 656-666 nm in water

    application(s)

    diagnostic assay manufacturing
    hematology
    histology

    storage temp.

    room temp

    SMILES string

    [Cl-].CN(C)c1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](/C)C

    InChI

    1S/C16H18N3S.ClH/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;/h5-10H,1-4H3;1H/q+1;/p-1

    InChI key

    CXKWCBBOMKCUKX-UHFFFAOYSA-M

    DESCRIPTION

    General description

    Methylene blue is used to stain animal cells to make their nuclei more observable. It is a component of staining media such as Wright′s stain and Jenner′s stain.
    Methylene blue, also known as methylthioninium chloride, is a photoactive phenothiazine dye.[1] It is a small molecular weight dye,[2] which is effective against Gram positive and Gram negative bacteria.[3]

    Application

    Methylene Blue solution has been used to stain human amniotic fluid stem cells to determine cell viability.[4] It has also been used to stain sections to determine the presence of mycobacteria.[5][6]
    Methylene blue is used to stain blood films/smears used in cytology and to stain RNA or DNA for viewing under the microscope or on hybridization membranes.

    SAFETY INFORMATION

    Storage Class

    10 - Combustible liquids

    wgk_germany

    WGK 1

    flash_point_f

    Not applicable

    flash_point_c

    Not applicable

    ppe

    Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter