Description
Malonate broth, 500G
NutriSelect® Plus, suitable for microbiology, pH 6.7±0.2 (25 °C)
PROPERTIES
sterility
non-sterile
Quality Level
200
form
powder
manufacturer/tradename
NutriSelect® Plus
technique(s)
microbe id | utilization test: suitable
microbiological culture: suitable
pH
6.7±0.2 (25 °C)
application(s)
food and beverages
water monitoring
microbiology
suitability
Klebsiella spp.
Salmonella spp.
nonselective and differential for Enterobacter spp.
nonselective and differential for Escherichia coli
nonselective and differential for coliforms
General description
Malonate broth is recommended for the differentiation of Enterobacter and Escherichia.
Application
Malonate broth has been used to evaluate the utilization of malonate by Enterobacter sakazakii.[1][2] It has also been used to differentiate between Cronobacter sakazakii and Cronobacter malonaticus isolates.[3]
Components
Components (g/L)
Ammonium sulfate, 2.00
Dipotassium phosphate, 0.60
Monopotassium phosphate, 0.40
Sodium chloride, 2.00
Sodium malonate, 3.00
Bromo thymol blue, 0.025
Ammonium sulfate, 2.00
Dipotassium phosphate, 0.60
Monopotassium phosphate, 0.40
Sodium chloride, 2.00
Sodium malonate, 3.00
Bromo thymol blue, 0.025
Preparation Note
Suspend 8 g of Malonate Broth in 1000 ml of distilled water. Dispense and sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 minutes. Avoid the addition of carbon and nitrogen from other sources.
Footnote
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
Legal Information
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
SAFETY INFORMATION
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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