Description
LB Broth (Miller) Liquid microbial growth medium
Liquid microbial growth medium
Synonym(s):
Luria Broth Miller
NACRES:
NA.85
PROPERTIES
grade
for molecular biology
Quality Level
400
sterility
sterile; 0.2 μm filtered
form
liquid
pH
6.5-7.2
storage temp.
room temp
suitability
nonselective for Escherichia coli
nonselective for coliforms
DESCRIPTION
General description
Miller′s LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates.
Application
Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added.
Features and Benefits
Miller′s LB liquid provides:
- Filter-sterilized, ready-to-use format
- Convenient package size
- Standard formulation
Components
10g/L Tryptone
10 g/L NaCl
5 g/L Yeast Extract
10 g/L NaCl
5 g/L Yeast Extract
SAFETY INFORMATION
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves