Kovac′s reagent for indoles, 100mL, suitable for microbiology

SKU:
67309-100ML-F
  • $102.00

    • Description

    Kovac′s reagent for indoles, 100mL

    suitable for microbiology

    Synonym(s):
    4-(Dimethylamino)benzaldehyde solution
    Empirical Formula (Hill Notation):
    C9H11NO
    CAS Number:
    100-10-7
    Molecular Weight:
    149.19
    Beilstein/REAXYS Number:
    4132845
    MDL number:
    MFCD00003381
    PubChem Substance ID:
    329760541
    NACRES:
    NA.85

    PROPERTIES

    agency

    according to ISO 16654:2001

    Quality Level

    200

    product line

    BioChemika

    shelf life

    limited shelf life, expiry date on the label

    composition

    4-(dimethlyamino)benzaldehyde, 50 g/L
    hydrochloric acid, 240 g/L
    isoamylic alcohol, 710 g/L

    technique(s)

    microbe id | specific enzyme detection: suitable

    application(s)

    agriculture
    clinical testing
    environmental
    food and beverages
    microbiology

    storage temp.

    2-8°C

    suitability

    Escherichia coli
    coliforms

    SMILES string

    [H]C(=O)c1ccc(cc1)N(C)C

    InChI

    1S/C9H11NO/c1-10(2)9-5-3-8(7-11)4-6-9/h3-7H,1-2H3

    InChI key

    BGNGWHSBYQYVRX-UHFFFAOYSA-N

    DESCRIPTION

    General description

    Kovac′s reagent is prepared by mixing p-dimethylaminobenzaldehyde, isoamyl alcohol and concentrated hydrochloric acid.[1] For identification of an organism, the formation of Indole from a tryptophan substrate is a useful diagnostic tool. Indole production is a crucial test in identification of Escherichia coli. After incubation, adding the reagent will help in determination if Indole that has been produced. The reagent turns red when reacts with Indole.[2]

    Application

    In the presence of oxygen, some bacteria, like E.coli, are able to split tryptophan into indole and alpha-aminopropionic acid. This reagent is for detecting the indole and identify the indole-positive and indole-negative microorganisms.
    Kovac′s reagent may be used for spot test for determination of indole by saturating filter paper with the reagent.[3] It may also be used for initial spot test for determination of indole followed by quantification by spectrophotometric assay and HPLC-UV/Vis-MS/MS methods.[4]

    SAFETY INFORMATION

    signalword

    Danger

    hcodes

    H226 - H290 - H314 - H317 - H332 - H335

    pcodes

    P210 - P233 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338

    Hazard Classifications

    Acute Tox. 4 Inhalation - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 3

    target_organs

    Respiratory system

    Storage Class

    3 - Flammable liquids

    wgk_germany

    WGK 1

    flash_point_f

    109.4 °F

    flash_point_c

    43 °C

    ppe

    Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter