KiCqStart Probe qPCR ReadyMix

for Bio-Rad, Cepheid, Eppendorf, Illumina, Corbett, and Roche systems, 250 reactions

Synonym: Kicqstart Probe qPCR ReadyMix

Properties

General description

KiCqStart Probe qPCR ReadyMix is an advanced qPCR reagent system for both fast and conventional PCR cycling protocols or instruments. It is a versatile and robust solution that provides the ultimate sensitivity and high PCR efficiency using a variety of fluorogenic probe chemistries, including TaqMan^® hydrolysis probes. KiCqStart Probe qPCR ReadyMix is provided as a 2X concentrated ready-to-use reaction cocktail that contains all required reaction components, except primers, probe(s), and DNA template. The light blue color of an inert tracer dye simplifies reaction assembly in white, or clear, plates and helps to minimize pipetting or mixing errors. It does not interfere with qPCR performance or affect the stability of the product.

A key component of KiCqStart Probe qPCR ReadyMix is an ultra-pure, processive thermostable DNA polymerase that is free of detectable E. coli DNA§. KiCqStart Probe qPCR ReadyMix is ideal for demanding qPCR applications such as bacterial pathogen detection where residual host DNA in typical recombinant enzyme preparations can limit assay sensitivity and obscure detection of low copy samples. The enzyme in KiCqStart Probe qPCR ReadyMix is combined with high avidity monoclonal antibodies to provide a stringent automatic hot-start that allows reaction assembly, and temporary storage, at room temperature prior to PCR amplification.

Application

KiCqStart^® Probe qPCR ReadyMix^™ has been used for gene expression analysis by qPCR.^[1]

Features and Benefits

• Assay results in as little as 33 minutes
• Highly efficient and sensitive real-time PCR results
• Little/no optimization required

Components

2X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dTTP), hot-start DNA polymerase, insert blue qPCR dye, and stabilizers.

packaging:
250 reactions* = 2 X 1.25 mL tubes
1250 reactions* = 10 X 1.25 mL tubes
5000 reactions* = 1 X 50 mL tube
*number of reactions based on a 20uL volume

Legal Information

Bio-Rad is a registered trademark of Bio-Rad Laboratories, Inc.

CFX384 is a trademark of Bio-Rad Laboratories, Inc.

CFX96 is a trademark of Bio-Rad Laboratories, Inc.

Chromo4 is a trademark of Bio-Rad Laboratories, Inc.

KiCqStart is a registered trademark of Qiagen Beverly Inc.

LightCycler is a registered trademark of Roche

Mastercycler is a registered trademark of Eppendorf AG

MiniOpticon is a trademark of Bio-Rad Laboratories, Inc.

Opticon is a trademark of Bio-Rad Laboratories, Inc.

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Rotor-Gene is a registered trademark of Qiagen GmbH

Scorpions is a registered trademark of Qiagen Group

SmartCycler is a registered trademark of Cepheid, Inc.

TaqMan is a registered trademark of Roche Molecular Systems, Inc.

Safety Information