Isopropyl-beta-D-thiogalactoside (IPTG) (1g)

SKU:
I6758-1G
  • $89.80

    • Description

    IPTG

    ≥99% (TLC), ≤0.1% Dioxane

    Synonym: IPTG, Isopropyl β-D-1-thiogalactopyranoside, Isopropyl β-D-thiogalactoside

    • CAS Number 367-93-1

    • Empirical Formula (Hill Notation) C9H18O5S

    • Molecular Weight 238.30

    •  Beilstein Registry Number 4631

    •  EC Number 206-703-0

    •  MDL number MFCD00063273

    Properties

    Quality Level PREMIUM
    description non-ionic
    assay ≥99% (TLC)
    impurities ≤0.1% Dioxane
    storage temp. 2-8°C

    Description

    Application

    IPTG is commonly used in cloning procedures that require induction of β-galactosidase activity. It is used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lacoperon in Escherichia coli. IPTG functions by binding to the lacI repressor and altering its conformation, which prevents the repression of the β-galactosidase coding gene lacZ.

    Other Notes

    Non-metabolizable galactose analog.

    Packaging

    1g in glass bottle

    Reconstitution

    A stock solution (0.1 M) is prepared by dissolving IPTG in water with subsequent sterile filtration of the solution. The final concentration of IPTG in indicator plates should be 0.2 mM.