HisTrap Fast Flow

SKU:
GE17-5255-01
  • $738.00

    • Description

    GE17-5255-01

    HisTrap Fast Flow

    Cytiva, 17-5255-01, pack of 5 × 5 mL

    Description

    General description

    HisTrap™ FF is a ready-to-use column, prepacked with precharged Ni Sepharose® 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC).

    Ni Sepharose® 6 Fast Flow consists of 90 μm beads of highly cross-linked agarose, to which a chelating ligand has been immobilized. This chelating ligand is charged with Ni2+ ions, the first-choice metal ion for purifying most histidine-tagged proteins. The negligible leakage of Ni2+ ions from the matrix ensures reliable capture of histidine-tagged proteins in repeated IMAC purifications.

    The high flow rates made possible by the Ni Sepharose® 6 Fast Flow matrix make HisTrap™ FF columns well suited for small scale-up. Capacity can be increased by connecting columns in series. HiTrap® IMAC FF is the product of choice when charging the medium with different metal ions for optimization of purification protocols.

    Features and Benefits

    • High binding capacity, approx. 40 mg/mL medium.
    • Negligible leakage of Ni2+.
    • Prepacked columns offer reliable and convenient time-saving purification of histidinetagged recombinant proteins.
    • Compatible with a wide range of reducing agents, detergents, denaturants, and other additives.

    Storage and Stability

    Store at 4 to 30 °C (20% Ethanol)

    Properties

    Related Categories Additional Affinity Purification, Molecular Biology, Plant Biotechnology, Plant Proteomics, Protein Purification More...
    shelf life   Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
    packaging   pack of 5 × 5 mL
    mfr. no.   Cytiva, 17-5255-01
    parameter   <4 mL/min flow rate (The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.)
      42 psi
    bed size   7 mm × 25 mm
    bed volume   1 mL
    column I.D.   7 mm
    matrix   6% cross-linked agarose
    particle size   45-165 μm
    average diameter   90 μm
    cleaning   2 - 14 (Ni2+-stripped medium.)
    working range   3 - 12 (Ni2+-stripped medium.)
    capacity   ~40 mg binding capacity(histidine-tagged protein)
    suitability   suitable for bioprocess medium