Description
Cytiva His Spintrap Columns
Properties
| shelf life | Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
| mfr. no. | Cytiva, 28-4013-53 |
| packaging | pkg of 50 x 100 μL |
| bed volume | 100 μL |
| matrix | highly cross-linked 6% agarose |
| avg. part. size | 34 μm |
| cleaning | 2 - 14 (Ni2+-stripped medium.) |
| working range | 3 - 12 (Ni2+-stripped medium.) |
| capacity | ≥40 mg binding capacity (histidine-tagged protein/ml medium)(Protein binding capacity is protein-to-protein dependent.) |
| ≥40 mg binding capacity (histidine-tagged protein/ml medium)(Protein binding capacity is protein-to-protein dependent.) |
Description
General description
His SpinTrap is a prepacked, single-use spin column for purifying histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC)
* See the section for licensing information in our terms and conditions.
Purification of unclarified samples on His SpinTrap columns minimizes loss of target protein caused by manual operations such as sample precentrifugation, transfer to centrifugation tubes, and collecting supernatant. In addition, loading unclarified sample directly to His SpinTrap columns reduces sample preparation time, which minimizes degradation and oxidation of sensitive target proteins.
His SpinTrap columns are used together with a standard microcentrifuge and one purification run takes approximately 10 min. For optimal performance, use His SpinTrap together with buffers prepared using His Buffer Kit.
The columns are prepacked with Ni Sepharose High Performance, which is precharged with nickel ions for capture of histidine-tagged proteins. Leakage of nickel ions from the Ni Sepharose High Performance medium is negligible.
Features and Benefits
• For simple, small-scale purification of histidine-tagged* proteins and rapid expression screening.
• Ni Sepharose High Performance in prepacked spin columns.
• Yields up to 750 μg pure protein per column.
• Allows direct purification of both clarified and unclarified cell lysates.
Storage and Stability
Store at 4 to 30 °C (0.15% Kathon CG)
Store at 4 to 30 °C (20% Ethanol)
Legal Information
Sepharose is a registered trademark of Cytiva
SpinTrap is a trademark of Cytiva
-
Nelson-JamesonCytiva Syringe Filters-Prefilter
SKU: 7163008
Contact Us For QuoteCytiva Syringe Filters-Prefilter SKU: 7163008 Mfr #: 6896-2504 Sold By: PK Quantity: 50/pk Pore Size: 0.2 µm Membrane/Filter -
RestekRaptor C18 EXP Guard Column Cartridge, 3PK
SKU: 930450252
$548.00Raptor EXP Guard Column Cartridges Product Name: Raptor C18 EXP Guard Column Cartridge Particle Size: 5 μm Size: 5 x 2.1 mm Free-Turn architecture lets you change cartridges by hand without... -
RestekRaptor ARC-18 EXP Guard Column Cartridge, UHPLC, 5 x 2.1 mm, 3pk
SKU: 9314U0252
$617.00Raptor EXP Guard Column Cartridges Free-Turn architecture lets you change cartridges by hand without breaking inlet/outlet fluid connections—no tools needed. Patented titanium hybrid ferrules can... -
RestekRaptor ARC-18 EXP Guard Column Cartridges (2.7um, 5mm, 3.0mm) 3pk
SKU: 9314A0253
$559.00Raptor EXP Guard Column Cartridges Free-Turn architecture lets you change cartridges by hand without breaking inlet/outlet fluid connections—no tools needed. Patented titanium hybrid...
