Description
Fructose Assay Kit
sufficient for 20 assays
This kit is for the quantitative, enzymatic determination of fructose in food and other materials.Fructose is phosphorylated by adenosine triphosphate (ATP) in the reaction catalyzed by hexokinase. Fructose 6-phosphate is converted to glucose 6-phosphate by phosphoglucose isomerase (PGI). Glucose-6-phosphate (G6P) is then oxidized to 6-phosphogluconate in the presence of nicotinamide adenine dinucleotide (NAD) in the reaction catalyzed by glucose-6-phosphate dehydrogenase (G6PDH). During this oxidation, an equimolar amount of NAD is reduced to NADH. The consequent increase in absorbance at 340 nm is directly proportional to fructose concentration.
EC Number:
200-333-3
NACRES:
NA.84
PROPERTIES
usage
sufficient for 20 assays
Quality Level
100
analyte chemical class(es)
sugars (fructose)
technique(s)
photometry: suitable
application(s)
food and beverages
general analytical
storage temp.
2-8°C
Kit Components Only
fructose standard 500 mg
glucose assay reagent 1 mL/vial
phosphoglucose isomerase 1 mL/vial
SAFETY INFORMATION
Pictograms
GHS08
Signal Word
Danger
Hazard Statements
H334
Precautionary Statements
P261 - P284 - P501
Hazard Classifications
Resp. Sens. 1
Storage Class Code
10 - Combustible liquids
WGK
WGK 3