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Fraser Broth, Base suitable for microbiology, NutriSelect Plus, 500G

SKU:
69198-500G
  • $177.00

    • Description

    Fraser Broth, Base suitable for microbiology, NutriSelect Plus, 500G

    Synonym(s):
    Listeria Selective Enrichement Broth, Base (acc. Fraser)
    NACRES:
    NA.85

    Quality Level

    200

    sterility

    non-sterile

    form

    powder

    shelf life

    limited shelf life, expiry date on the label

    composition

    casein enzymic hydrolysate, 5.0 g/L
    disodium phosphate dihydrate, 12.0 g/L
    esculin, 1.0 g/L
    lithium chloride, 3.0 g/L
    meat extract, 5.0 g/L
    peptic digest of animal tissue, 5.0 g/L
    potassium dihydrogen phosphate, 1.35 g/L
    sodium chloride, 20.0 g/L
    yeast extract, 5.0 g/L

    manufacturer/tradename

    NutriSelect® Plus

    technique(s)

    microbiological culture: suitable

    final pH

    7.2±0.2 (25 °C)

    application(s)

    agriculture
    environmental
    food and beverages
    pathogen testing
    sample preparation
    microbiology

    suitability

    selective and differential for Listeria spp.

    Fraser Broth Base with added supplement is recommended by ISO Committee for primary and secondary enrichment medium, for the isolation and enumeration of Listeria monocytogenes from food and animal feeds

    Preparation Note

    Suspend 57.35 grams of dehydrated medium in 1000 ml distilled water. Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C and aseptically add rehydrated contents of 1 vial of Fraser Selective Supplement (Cat. No. 18038) and 2 vials of Fraser Supplement (Cat. No. 90836) to 1000 ml medium for primary enrichment or 1 vial of each to 500 ml medium for secondary enrichment. Mix well and dispense as desired.
    Warning : Lithium chloride is harmful. Avoid bodily contact and inhalation of vapours. On contact with skin wash with plenty of water immediately.

    Footnote

    We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
    The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

    Legal Information

    GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
    NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

    SAFETY INFORMATION

    Storage Class Code

    11 - Combustible Solids

    WGK

    WGK 3

    Flash Point(F)

    Not applicable

    Flash Point(C)

    Not applicable

    Personal Protective Equipment

    dust mask type N95 (US), Eyeshields, Gloves