Description
Fast Green FCF, 25mg
analytical standard
Synonym(s):
Food green 3
About This Item
Linear Formula:
C37H34N2O10S3Na2
CAS Number:
2353-45-9
Molecular Weight:
808.85
Colour Index Number:
42053
Beilstein/REAXYS Number:
5718212
EC Number:
219-091-5
MDL number:
MFCD00013053
UNSPSC Code:
85151701
PubChem Substance ID:
329761116
E Number:
E143
NACRES:
NA.24
Properties
grade
analytical standard
Quality Level
100
assay
≥98.0% (HPLC)
technique(s)
HPLC: suitable
gas chromatography (GC): suitable
mp
290 °C (dec.) (lit.)
application(s)
cleaning products
cosmetics
food and beverages
personal care
format
neat
SMILES string
[Na+].[Na+].CCN(Cc1cccc(c1)S([O-])(=O)=O)c2ccc(cc2)C(=C3/C=CC(\C=C3)=[N+](\CC)Cc4cccc(c4)S([O-])(=O)=O)\c5ccc(O)cc5S([O-])(=O)=O
InChI
1S/C37H36N2O10S3.2Na/c1-3-38(24-26-7-5-9-33(21-26)50(41,42)43)30-15-11-28(12-16-30)37(35-20-19-32(40)23-36(35)52(47,48)49)29-13-17-31(18-14-29)39(4-2)25-27-8-6-10-34(22-27)51(44,45)46;;/h5-23H,3-4,24-25H2,1-2H3,(H3,41,42,43,44,45,46,47,48,49);;/q;2*+1/p-2
InChI key
RZSYLLSAWYUBPE-UHFFFAOYSA-L
Description
General description
Fast Green FCF is a synthetic organic[1] dye[2], which belongs to the triphenylmethane group.[2] It can be found in a wide range of applications in food and beverage industry.[1] It can also be used in scientific research for the staining of proteins and identification of extracellular recording or injection sites in electrophysiological experiments.[1]
Application
Fast Green FCF dye is used for protein staining in IEF, native PAGE and SDS-PAGE. Fast Green staining is linear over a wider range of protein concentrations than Brilliant Blue R. After electrophoresis, fixing the proteins in the gel is recommended for maximum sensitivity. The gel can then be stained for 2 hr with 0.1% Fast Green FCF in either 30% (v/v) ethanol, 10% (v/v) acetic acid or in 7% acetic acid. The gel can be destained with either 30% (v/v) ethanol, 10% (v/v) acetic acid, or 7% acetic acid. The stained gel can be scanned at 625 nm. The detection sensitivity is approx. 30% that with Brilliant Blue R.
Refer to the product′s Certificate of Analysis for more information on a suitable instrument technique. Contact Technical Service for further support.
Packaging
Bottomless glass bottle. Contents are inside inserted fused cone.
Safety Information
signalword
Warning
hcodes
H335,H341
pcodes
P202 - P261 - P271 - P280 - P304 + P340 + P312 - P308 + P313
Hazard Classifications
Muta. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
11 - Combustible Solids
wgk_germany
WGK 2
flash_point_f
352.4 °F - closed cup
flash_point_c
178 °C - closed cup