Description
Fast Blue B Salt Dye content ~95 %, 10G
Synonym: o-Dianisidine bis(diazotized) zinc double salt, Azoic Diazo No. 48, DBB, Diazo Blue B, Naphthanil Diazo Blue B
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CAS Number 14263-94-6
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Linear Formula C14H12N4O2Cl2 · ZnCl2
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Molecular Weight 475.47
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Colour Index Number 37235
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Beilstein/REAXYS Number 1499067
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EC Number 238-153-2
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MDL number MFCD00075203
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PubChem Substance ID 24894113
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NACRES NA.47
Properties
Related Categories | Aflatoxins, Analytical Reagents, Analytical/Chromatography, Cell Biology, Derivatization Reagents,
Derivatization Reagents TLC, E-F, Hematology and Histology, Stains & Dyes, A to Z, Stains and Dyes, TLC Reagents, D-F, TLC Visualization (Staining) Reagents (alphabetic sort), TLC Visualization (Staining) Reagents (by application)
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Quality Level | 200 |
description | Technical grade |
form | powder |
composition | Dye content, ~95% |
application(s) | hematology: suitable |
- | histology: suitable |
mp | >300 °C (lit.) |
solubility | H2O: 1 mg/mL |
Featured Industry | Diagnostic Assay Manufacturing |
storage temp. | 2-8°C |
SMILES string | [Cl-].[Cl-].Cl[Zn]Cl.COc1cc(ccc1[N+]#N)-c2ccc([N+]#N)c(OC)c2 |
InChI | 1S/C14H12N4O2.4ClH.Zn/c1-19-13-7-9(3-5-11(13)17-15)10-4-6-12(18-16)14(8-10)20-2;;;;;/h3-8H,1-2H3;4*1H;/q+2;;;;;+2/p-4 |
InChI key | GPPKNJIWDULNQH-UHFFFAOYSA-J |
Application
• Fast Blue B has been used in the acid phosphatase test in Clostridium perfringens.[1]
• It has been used as a spraying agent in thin layer chromatography for the identification of separated components.[2]
• It has also been used for the determination of the lipase activity.[3][4]
Packaging
10, 100 g in glass bottle
Biochem/physiol Actions
Fast blue B is used for the determination of acetylcholinesterase activity. Acetylcholinesterase converts 1-naphthyl acetate to 1-naphthol, which reacts with fast blue B to form a purple-coloured diazonium dye.[5] Similarly, fast blue B is also used for α- and β-glucosidase activity.[6]